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Longer pulse length for conducting samples?

3 0 7k
answers votes views
Hi, my 1H 90 deg pulses are always longer when I work with my samples that are electrically conducting. Is this a/the re...
Posted: Santhosh 1 / Updated: glass lizard 31 Aug 12 '12 at 12:24

qfil: baseline correction

3 0 5k
answers votes views
"the t1 dimensio was processed using the baseline correction mode “qfil” in the XWINNMR software" Does anyone know abou...
Posted: Frey 61 / Updated: Kirk Marat 711 Sep 19 '12 at 13:11

2D NOESY filtered/edited experiments

3 0 7k
answers votes views
what are the name for 2D NOESY filtered/edited experiments like F1fF2f, F1fF2e, F2f, F1eF2e in Bruker 700MHz ?. Here is ...
Posted: ambrish 1 / Updated: marcs 11 Nov 28 '12 at 08:13

Long-range 1H-{15N} coupling (HMBC)

3 1 8k
answers votes views
Hello All I have two examples of what appears to be 6J coupling between 1H and 15N using gradient HMBC experiments with...
Posted: Craig 41 / Updated: Evgeny Fadeev 577●1 Feb 16 '13 at 15:17

Varian VNMRJ 2.2D standard experiments

3 1 7k
answers votes views
Hey folks, I was wondering if anyone else has had any issues with the experiments/pulse sequences that came standard in ...
Posted: CaymanDave 11 / Updated: Bharathwaj 112 Nov 09 '12 at 08:16

Where to by RT-Shim set + power supply?

3 0 4k
answers votes views
Hi, I am searching for an RT-Shim set with a manual power supply (not controlled by software like topspin) for a WB mag...
Posted: fid 139 / Updated: Kirk Marat 711 Dec 06 '12 at 08:09

NQR technique

3 0 4k
answers votes views
Hi.I would like to run NQR test.I have some questions about it.are there any machines for this technique?if yes,please t...
Posted: shideh bb 1 / Updated: Elena Kurenkova 1 Jun 10 '13 at 00:34

Gsim 2D transformation (TopSpin)

3 0 4k
answers votes views
Hello. Anybody can use Gsim for 2D liquid spectra transformation? I can not transform correctly the TopSpin .ser files....
Posted: Attila 1 / Updated: Ulrich Haunz 210 Mar 11 '13 at 01:51

35Cl NMR spectra

3 0 7k
answers votes views
Hi, I am new here! I am working on Bruker 600 MHz over 10 years, but now I have problem to get 35Cl NMR spectrum. I use...
Posted: gugica 1 / Updated: Tony Bielecki 131 Jul 10 '13 at 07:32

Selectively coupled 13C NMR

3 0 9k
answers votes views
Is it possible to run a 13C spectra in which one carbon is coupled (with 1H) and remaining carbons are decoupled, using ...
Posted: praveen 1 / Updated: sekhar Talluri 621 Oct 27 '13 at 04:20

can topspin 3.2 window version open broker fid file?

3 0 4k
answers votes views
I installed the Topspin 3.2 pl 3 on windows 7, but when I am trying to open my NMR data it can only show process data fi...
Posted: shark 1 / Updated: John Hollerton 96 Jan 23 '14 at 09:36

Topspin 3.2.5 queue experiment

3 0 10084
answers votes views
Does anyone know how to make Topspin to queue experiments? In my recently installed Topspin somehow the queue function i...
Posted: shark 1 / Updated: Arkadiusz Leniak 238 Jun 05 '14 at 05:07

RGA setting too high on Bruker Avance 300

3 0 7k
answers votes views
I'm using Bruker Avance 300 spectrometer (Topspin processing software). When using "RGA" GR value jumps up to 3000 and m...
Posted: Avance300 1 / Updated: John Hollerton 96 Sep 10 '14 at 04:07

19F full range homodecoupling

3 0 8k
answers votes views
Greetings, this is my first question in this wiki :) I wonder if there's possibility to obtain full homo decoupled spe...
Updated: Arkadiusz Leniak 238 Jun 16 '14 at 00:14

Topspin 2.1 error message with Windows 7 OS

3 0 8k
answers votes views
Hi all, I have been running TopSpin 2.1 on my Windows 7 32-bit OS for the last few years and now am getting this annoyin...
Posted: Olivia 1 / Updated: Ulrich Haunz 210 Nov 14 '14 at 03:16

problem with large dosy files

3 0 3k
answers votes views
Hello, I run DOSY expt successfully but my dosy files are very massive and they take up large amounts of disk space. Ho...
Updated: elham 3 Jan 16 '15 at 07:57

vnmrj spectra in mnova

3 0 12484
answers votes views
I have been trying to open varian vnmrj spectra in mnova software to workup the spectra, but I keep getting error messag...
Posted: Carolyn Shetter 1 / Updated: Ulrich Haunz 210 Aug 13 '14 at 05:09

Absence of some signals on 1H and 13C spectra of polyaromatic carboximidate

3 0 8k
answers votes views
Hi, I have encountered a strange behaviour of some signals (they are absent) in a series of polycyclic aromatic carboxi...
Posted: Arkadiusz Leniak 238 / Updated: zablociak 21 Mar 23 '15 at 12:11

Why so much spam?

3 3 3k
answers votes views
Hi, I really like this forum and I am harrowed to see it so spammed. We should find a way to end this(maybe more admins...
Posted: gravatar 83 / Updated: Ulrich Haunz 210 Oct 24 '14 at 07:09

Inverse FT of NUS data

3 0 4k
answers votes views
Does anyone know the way to create a full ser file from Bruker NUS processed data? I want to do this so that others (wi...
Posted: John Hollerton 96 / Updated: Pete Gierth 401 Dec 05 '14 at 11:19

19F 2D experiments

3 0 7k
answers votes views
Hi, I would like to run some 19F NOESY, and 19F-1H correlation experiments on a totally fluorinated sample (no H) with a...
Posted: AnneBaudouin 1 / Updated: RuudA 11 Nov 02 '14 at 07:44

coupling constants

3 0 3k
answers votes views
Hi, does anybody know 1H-79Br coupling constants? I found in literature only 13C-79Br coupling constants! Thank you, Mar...
Posted: gugica 1 / Updated: Arkadiusz Leniak 238 Dec 07 '14 at 02:28

How to optimally choose a 1/4J 1H-15N delay for INEPT?

2 1 7k
answers votes views
Why is it often the case that when transferring magnetization from 1H to 15N, the 1/4J time is often a little less than ...
Posted: Scott Robson 156 / Updated: Evgeny Fadeev 577●1 Apr 14 '10 at 16:33

finding sample data for practicing 2D 1H assignment

2 1 6k
answers votes views
as I have already started to assign a small peptide, I need sample data set for practicing 2d h1 assignment, could you p...
Posted: niloofar niknam 26 / Updated: jkurutz 131 Dec 15 '09 at 05:00

calculating power level with stdisp

2 2 9k
answers votes views
For some reason the shape tool has some sort of glitch that I can't calculate a power level for a wurst 10 ms pulse 180 ...
Posted: roseyp 46 / Updated: djh 1 Aug 11 '11 at 04:14

Please suggest model proteins and peptides for NMR

2 1 6k
answers votes views
do you know any model proteins except lysozyme suitable for nmr experiments? ...
Posted: niloofar niknam 26 / Updated: Markus Seeliger 11 Aug 17 '10 at 13:16

How to process gNhsqc_IPAP.c data with nmrPipe?

2 0 13805
answers votes views
Hello, I know that this problem has been discussed a number of times on the nmrPipe newsgroup and there are some helpfu...
Updated: Evgeny Fadeev 577●1 Mar 03 '10 at 13:11

Helium recovery system

2 1 7k
answers votes views
How do I configure a helium recovery system that won't perturb my magnet's stability? I'm concerned that just hooking ...
Posted: jkurutz 131 / Updated: Jerry Hirschinger 199 Jul 23 '14 at 10:45

What may cause lock level depend on pfgon setting in vnmr?

2 1 6k
answers votes views
Hello, I've been working through the probe performance tests and noticed that when I change pfgon='yyy' to pfgon='nyn' l...
Posted: Evgeny Fadeev 577●1 / Updated: Jerry Hirschinger 199 Sep 28 '10 at 12:12

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

2 1 13987
answers votes views
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Posted: Evgeny Fadeev 577●1 / Updated: Justin Frank 1 Jun 27 '16 at 19:27

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

2 0 20424
answers votes views
By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry. Also - ...
Posted: Evgeny Fadeev 577●1 / Updated: SankarampadiAravamudhan 91 Mar 17 '11 at 01:01

Are there alternatives to MOLMOL for visualization of NMR structure ensemebles?

2 0 5k
answers votes views
Hi folks, I've been using Molmol, but found significant issues when working with a non-standard structure. For example...
Updated: Evgeny Fadeev 577●1 Mar 02 '10 at 08:05

How to translate Bruker shaped pulse statements to Varian?

2 2 7k
answers votes views
I was wondering how to add a shaped-pulse to a standard fHSQC pulse program on a Varian? ...
Posted: dcapraro 21 / Updated: Evgeny Fadeev 577●1 Mar 02 '10 at 08:15

Are there tools that automate obtaining RDC values and accuracy from NMR data?

2 1 6k
answers votes views
Hello. I was wondering if there are any software programs to extract RDC values, and their associated errors from proces...
Posted: Elio Cino 31 / Updated: bernie o'hare 111 Mar 13 '10 at 10:30

Where can I find information about the basics of Solid State NMR Pulse Sequences?

2 1 8k
answers votes views
Help me in getting the books and articles related to know the basics of Pulse Sequence for Solid State NMR. ...
Posted: Padma Prasad Karthikeyan 11 / Updated: bernie o'hare 111 Mar 13 '10 at 10:29

How do you determine error on the peak position?

2 0 12608
answers votes views
Hello, could anyone suggest a method to determine error (maybe standard deviation or some other estimate) of a peak posi...
Posted: Evgeny Fadeev 577●1 / Updated: sekhar Talluri 621 Mar 14 '10 at 08:46

How do you calculate the % or degree of acrylation of a polymer using integrated NMR peaks?

2 1 21394
answers votes views
Hello, I'm trying to use an NMR spectrum to define/calculate a "degree of acrylation" for a PLA-PEG-PLA block copolymer ...
Updated: juxtabo 11 Apr 11 '10 at 10:48

How to confirm structure of propane by NMR?

2 2 5k
answers votes views
I will really need your help. I am chemist working in the oil buisness and I am interesting is it possible to analyze pr...
Posted: Silvija 21 / Updated: ron daedalus 11 Apr 21 '10 at 09:18

How to best correlate Deuterium with other nuclei by NMR?

2 0 6k
answers votes views
Hello, I've been asked to solve a problem of assigning a positon of deuteration on a molecule, where structure of the de...
Posted: Evgeny Fadeev 577●1 / Updated: kiscs kiscs 21 May 17 '10 at 16:43

BRUKER Fid data ordering

2 2 17406
answers votes views
Hi guys : This post is related to : qa.nmrwiki.org/question/83/where-to-find-technical-information-on-varian-and-bruke...
Updated: j 131 May 25 '10 at 11:45

MODULE for RDC

2 1 6k
answers votes views
I'm trying to analyze my RDC data using Module. But I'm using a crystal structure instead of NMR. Can somebody pls advis...
Posted: shub 31 / Updated: Evgeny Fadeev 577●1 Jul 28 '10 at 11:09

How to create synthetic procpar files for VNMRJ?

2 2 8k
answers votes views
Hi guys : Im trying to make a procpar for an FID, I know most of the parameters of importance, and have the schema of th...
Posted: j 131 / Updated: David Horita 31 Nov 09 '12 at 06:43

Chemical shift and spin rate

2 1 5k
answers votes views
For solid state NMR: What can cause the line position shift depending on the spin rate? ...
Posted: KateSTR 11 / Updated: Evgeny Fadeev 577●1 Jun 21 '10 at 19:12

Looking for sample spectra....

2 1 6k
answers votes views
Hi guys : Is there a public database of sample Bruker/Varian NMR spectra ? I want to look at a sample 4D SER file ......
Posted: j 131 / Updated: Evgeny Fadeev 577●1 Jul 07 '10 at 14:41

What is your test for the feasibility of protein structure determination?

2 1 6k
answers votes views
How do you evaluate protein samples for the feasibility to solve 3D structure? For example if you record a test HSQC se...
Posted: Evgeny Fadeev 577●1 / Updated: Ilango 171 Jul 21 '10 at 12:35

How do you port pulse sequences to different field strength?

2 0 17270
answers votes views
Hi, when you set up a pulse sequence at a different magnet field strength - starting with an existing set of parameters ...
Posted: Evgeny Fadeev 577●1 / Updated: Kirk Marat 711 Aug 07 '10 at 09:07

Bruker Avance pulse sequences - where to find if not in TopSpin?

2 2 40893
answers votes views
I used to use Varian Inova systems with BioPack all the time, and now I'm using a Bruker Avance-III system geared for Ch...
Posted: jkurutz 131 / Updated: Charlie Fry 91 Aug 08 '10 at 09:34

How much benefit to expect from best NMR tubes?

2 0 7k
answers votes views
Hello, how much benefit in resolution and signal-to-noise will we get when using 800 MHz rated tubes (on the 800 MHz ins...
Posted: Evgeny Fadeev 577●1 / Updated: sekhar Talluri 621 Sep 09 '10 at 08:31

Different proton Carrier frequencies

2 1 6k
answers votes views
Hi, I am a new member to this community. I would like to set up an experiment, in which I have to set up proton carrier ...
Posted: kmd 11 / Updated: Evgeny Fadeev 577●1 Aug 19 '10 at 10:27

Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe?

2 0 5k
answers votes views
If I'm running relaxation experiments (15N) for R1, R2, and hetnoe on a double labeled (2H/15N) sample, do I need to do ...
Posted: anita 31 / Updated: roseyp 46 Sep 01 '10 at 12:55
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