1
|
do you know any model proteins except lysozyme suitable for nmr experiments? |
|
1
|
My understanding is that you either want to learn how to analyze NMR data or test a new experiment on a well-behaving sample. If you are interested in small-medium sized proteins in liquid phase then I recommend human ubiquitin. It is a very stable well-folded protein with five beta strands and two helical motifs. Beta strands and helices have different NOE patterns, chemical shifts of the backbone residues, and some scalar couplings so you can learn how to recognize those using the ubiquitin sample. There is a lot of data published on ubiquitin, including Biomolecular NMR Data Bank - BMRB. Also there is a great ubiquitin NMR resource website built by Richard Harris at the University College of London that has 17 2D and 3D data sets together with nmrPipe processing scripts. |
|
1
|
There are a few to consider: ubiquitin, maltose binding protein - they may even be available commercially as protein standards |
Even though original poster did not explain what is the purpose of the modeling, it would still be great to answer this question broadly: e.g. for solids, liquids, structrue, dynamics, intermolecular interactions, chemical exchange, etc. It will be very valuable for the future visitors of this page. - Evgeny Fadeev (Jan 23 '10 at 13:05)
another suggestion for the answer: mention a model system with a paramagnetic center - good for picking up PRE or exploitation of pseudocontact paramagnetic shifts. - Evgeny Fadeev (Jan 23 '10 at 13:12)