Tagged questions

assignment of 12 kd protein

6 1 5k
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Dear NMR Wikiers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone ...
Posted: sri 71 / Updated: Thomas Garner 81 Aug 30 '11 at 07:11

Line broadening in nmr

5 0 7k
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Hello can some body help me in understanding the line broadening effect in NMR? I am working with protein and small mol...
Posted: fatima A 1 / Updated: Evgeny Fadeev 5771 Oct 12 '11 at 07:25

peak missing in nh hsqc protein nmr

4 0 16494
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Dear NMR wikiers I am assigning 95 aminoacid residue protein . I can see 76 cross peaks in N H HSQC on 700 vnmr cryob ...
Updated: sri 71 Aug 25 '11 at 00:05

Spectra from BMRB Chemical shifts

4 0 6k
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Is it possible to generate a spectrum from BMRB chemical shifts? I want to generate a spectrum to use as a reference fo...
Posted: Andreas 1 / Updated: delaglio 1361 Dec 11 '11 at 14:15

NMR of proteins

3 0 4k
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I need informations about nuclear magnetic resonance of proteins with figures. ...
Posted: f_tahoori 6 / Updated: Justin Frank 1 Jun 27 '16 at 20:47

NMR experiments to assign aromatic sidechains?

3 1 7k
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Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'...
Posted: anita 31 / Updated: roseyp 46 Feb 22 '11 at 09:38

Aromatic sidechain assignment pulse sequences for Varian?

3 0 4k
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Hi, someone asked a similar question before, but I'd like to know: Are there readily available varian pulse sequences t...
Posted: Magnethead 42 / Updated: Dummy 31 Nov 19 '10 at 06:28

Best pulse sequence for HSQC in Guanidine-HCl

3 1 7k
answers votes views
Hello, I would like to acquire an HSQC spectrum of an 15N-labeled 12 kDa protein unfolding (over the course of hours) i...
Posted: vadim 23 / Updated: Pete Gierth 401 Oct 30 '10 at 06:29

RDC gel preparation

3 0 5k
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I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the fol...
Posted: andrewL 1 / Updated: Andrew Fowler 96 Apr 24 '13 at 07:06

Please suggest model proteins and peptides for NMR

2 1 4k
answers votes views
do you know any model proteins except lysozyme suitable for nmr experiments? ...
Posted: niloofar niknam 26 / Updated: Markus Seeliger 11 Aug 17 '10 at 13:16
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