caluclation of duterium percentage of randomly deuterated protein

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Dear NMR WIKIERS ,

IS THEIR ANY NMR EXPERMENT ON 700 VNMR TO KNOW THE duterium percentage of randomly deuterated protein WHICH PREPARED USING 90 PERCENT D20 AND 10 PERCENT H2O ?

Regards

SRI

deuteium percentage of protein

asked Jun 21 '11 at 00:10

sri
71

I don't think so. Use mass spectroscopy if you can is the easiest way... - Yoan Monneau (Jun 21 '11 at 01:25)

thank you - sri (Jun 21 '11 at 01:35)

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I think you are better off forgetting NMR methods for determining the deuteration percentage of your protein. Mass spec (using simple MALDI or ESI) is by far the quickest and easiest method. Simply calculate the expected molecular weight of your protein in a fully-protonated state and in a fully-deuterated state and then apply the following formula:

Average % deuteration = 100 * (experimental MW from mass spec - theoretical fully protonated MW) / (theoretical fully deuterated MW - theoretical fully protonated MW)

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answered Jun 21 '11 at 10:05

ChemMJW
99

thank alot . I am new to biomoleculer nmr - sri (Jun 21 '11 at 18:59)

Hi Sri, if you like the answer, please mark it as "accepted" - just click the checkmark below the votes. - Evgeny Fadeev (Jun 21 '11 at 20:32)

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Asked: Jun 21 '11 at 00:10

Seen: 4,550 times

Last updated: Jun 21 '11 at 20:32

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