Tagged questions

protein-protein complex structure determination

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Dear Friends, I am working on a protein-protein complex using 700MHz VARIAN NMR . I have done HSQC perturbation studi...
Posted: Arun Gupta 11 / Updated: service email start 11 Mar 15 '12 at 17:40

Isotope Filtered NOESY

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I'm an NMR newbie. I know isotope filtering can be used to study a complex under slow exchange, but for fast-exchange, i...
Posted: Student Tim 1 / Updated: Eugene DeRose 41 Feb 28 '12 at 12:56

Varian Biopack pulse sequences for intermolecular NOEs

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Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the mom...
Posted: acalab 21 / Updated: Evgeny Fadeev 5771 Aug 10 '10 at 16:52

Will intermediate exchange give poor half filtered NOE

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I have recorded 2D half filtered NOE experiments on a protein-protein complex and got very poor quality data, why would ...
Posted: TomgA20 61 / Updated: Evgeny Fadeev 5771 Jul 15 '10 at 08:17

2D pulse sequences for NMR of protein/peptide complexes?

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Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone ...
Posted: acalab 21 / Updated: Evgeny Fadeev 5771 Jul 26 '10 at 09:25
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