Tagged questions

Please suggest model proteins and peptides for NMR

2 1 4k
answers votes views
do you know any model proteins except lysozyme suitable for nmr experiments? ...
Posted: niloofar niknam 26 / Updated: Markus Seeliger 11 Aug 17 '10 at 13:16

NMR experiments to assign aromatic sidechains?

3 1 7k
answers votes views
Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'...
Posted: anita 31 / Updated: roseyp 46 Feb 22 '11 at 09:38

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

2 1 10714
answers votes views
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Posted: Evgeny Fadeev 5771 / Updated: Justin Frank 1 Jun 27 '16 at 19:27

What is the minimum information required to calculate a structure with cyana?

1 1 6k
answers votes views
Hi guys : I have several spectra, and wanted to test out what data I do , and do not need, in order to effectively get a...
Posted: j 131 / Updated: Bharathwaj 112 Jun 28 '10 at 19:43

2D pulse sequences for NMR of protein/peptide complexes?

0 1 2k
answers votes views
Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone ...
Posted: acalab 21 / Updated: Evgeny Fadeev 5771 Jul 26 '10 at 09:25

What is your test for the feasibility of protein structure determination?

2 1 4k
answers votes views
How do you evaluate protein samples for the feasibility to solve 3D structure? For example if you record a test HSQC se...
Posted: Evgeny Fadeev 5771 / Updated: Ilango 171 Jul 21 '10 at 12:35

Examples of protein degradation eg HSQC?

1 1 3k
answers votes views
Anyone know any websites or papers that show protein degradation in an HSQC for example? I wanted to know what to look ...
Posted: madge 11 / Updated: Evgeny Fadeev 5771 Aug 08 '10 at 16:13

Best pulse sequence for HSQC in Guanidine-HCl

3 1 7k
answers votes views
Hello, I would like to acquire an HSQC spectrum of an 15N-labeled 12 kDa protein unfolding (over the course of hours) i...
Posted: vadim 23 / Updated: Pete Gierth 401 Oct 30 '10 at 06:29

cs rosetta compilation for linux 32

1 1 4k
answers votes views
Hi guys : I was wondering if anyone knows where I can find CS-Rosetta compiled for 32 bit linux..... Im having trouble ...
Posted: j 131 / Updated: Evgeny Fadeev 5771 Jan 13 '11 at 12:27

assignment of 12 kd protein

6 1 5k
answers votes views
Dear NMR Wikiers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone ...
Posted: sri 71 / Updated: Thomas Garner 81 Aug 30 '11 at 07:11
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