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Hi, I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow a few NOEs without running a whole 2D experiment. Is there any sequence in Varian BioPack that I could use for that? I cannot achieve water suppression with the standard 'Noesy1D' sequence and all the other seem to not allow to selectively irradiate only one peak. Maybe it is just a matter of right parameters but I don't know how to set them properly - if so I will appreciate any advice. Thanks |
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hi first you can run 1D preset with click 'do scout' and achieve good water-suppression, than you go to experiment,and convert current parameters (that is your preset parameters) in any 1D NOESY exp. and select sw -1 to- 12 or more than run you 1D noesy exp. may be it help you. sujeet Thank you for the suggestion. Unfortunately it seems that BioPack doesn't allow to jump between experiments while keeping parameters so I'll have to try to deactivate it first. I'm also afraid that presat may be not sufficient for my sample with H2O content at 90%. I'll try anyway and let you know - slawojek (Sep 17 '13 at 08:26) |
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Do you actually need water suppression? For example, I've found that the 1D gradient based NOE is quite capable of selectively irradiating protons that are far from the solvent peak. If your target protons don't overlap with H20, you might be able to get away skipping the solvent supression. |
