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There are many Software Tools available for that purpose. What are the problems there (if there are some)?
Do you think...
Updated:
FAS
1
Dec 08 '16 at 07:53
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Hi All,
I am trying to design a 1D 31P pH titration experiment for a protein. I have been trying to identify a good buf...
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I am running a 15N HSQC on a protein sample which is in a 100% deuterium oxide solvent. The command rga in TopSpin gives...
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Hello all,
I have used stretched polyacrylamide gels successfully many times to collect RDC data for proteins. The las...
Updated:
ChemMJW
99
Dec 06 '13 at 19:10
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Hi,
I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow ...
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I upload my protein on csrosetta server for generating structures. I have provided TALOS data file.
Its already two days...
Posted:
anup
1
Jun 04 '13 at 06:09
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Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2?
...
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I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the fol...
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views |
Is it possible to generate a spectrum from BMRB chemical shifts?
I want to generate a spectrum to use as a reference fo...
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votes |
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Hello friends ,
my target Protein is existing as multimer (i.e Monomer,dimer,tetrameter ) before binding to the peptid...
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Hello
can some body help me in understanding the line broadening effect in NMR? I am working with protein and small mol...
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Dear NMR wikiers
I would like to take c13 filter noesy experment ( this experment explore residues of the interface of...
Updated:
sri
71
Jul 05 '11 at 10:56
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Dear NMR wikiers
I am assigning 95 aminoacid residue protein . I can see 76 cross peaks in N H HSQC on 700 vnmr cryob ...
Updated:
sri
71
Aug 25 '11 at 00:05
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Dear nmr wikiers
could you please let me know is their any nmr experment to identification of Dimer and tetramer equil...
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Dear NMR WIKIERS ,
IS THEIR ANY NMR EXPERMENT ON 700 VNMR TO KNOW THE duterium percentage of randomly deuterated prote...
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Dear NMR Wikiers !
I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone ...
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Hi guys : I was wondering if anyone knows where I can find CS-Rosetta compiled for 32 bit linux..... Im having trouble ...
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Hello,
I would like to acquire an HSQC spectrum of an 15N-labeled 12 kDa protein unfolding (over the course of hours) i...
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Hi, someone asked a similar question before, but I'd like to know:
Are there readily available varian pulse sequences t...
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When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with:
15N
13C
both
...
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How do you evaluate protein samples for the feasibility to solve 3D structure?
For example if you record a test HSQC se...
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views |
Anyone know any websites or papers that show protein degradation in an HSQC for example? I wanted to know what to look ...
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Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone ...
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Hi guys : I have several spectra, and wanted to test out what data I do , and do not need, in order to effectively get a...
Posted:
j
131
/ Updated:
Bharathwaj
112
Jun 28 '10 at 19:43
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I had two problems like this one recently:
have protein (in solution) of known general structure
resonance assignments...
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I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
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Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'...
Posted:
anita
31
/ Updated:
roseyp
46
Feb 22 '11 at 09:38
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I need informations about nuclear magnetic resonance of proteins with figures.
...
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do you know any model proteins except lysozyme suitable for nmr experiments?
...
