Unanswered questions

why we are seeing negative PREs in paramagnetic relaxation enhancement experiment?

0 1 3k
answers votes views
Hi NMR Wiki users, I'm using two point method(Marius Clore and Junji Iwahara methods) to measure the PREs, but in my ex...
Posted: Kris 11 Dec 11 '12 at 07:48

Protein-Protein Interactions

1 0 3k
answers votes views
Hi i'm a newbie in NMR and I have 2 proteins, A protein is about 12KDa, B protein is about 45 KDa,i know that A protein ...
Posted: Guch 1 / Updated: Justin Frank 1 Jun 27 '16 at 22:46

Amplifier problem

4 0 8k
answers votes views
Hi.We have a 500 MHz AVANCE-DRX spectrometer and I have a question about it .How we can understand that the amplifier ha...
Posted: shokoufeh karimnia 1 / Updated: Nonai 1 May 22 '14 at 00:45

Where to by RT-Shim set + power supply?

3 0 3k
answers votes views
Hi, I am searching for an RT-Shim set with a manual power supply (not controlled by software like topspin) for a WB mag...
Posted: fid 139 / Updated: Kirk Marat 711 Dec 06 '12 at 08:09

NMRPipe commands

1 0 4k
answers votes views
I would like to know what is the command being used to read the parameter files of bruker when 'Read Parameter' button i...
Posted: Ved Roy 1 / Updated: TJCarruthers 31 Mar 24 '13 at 18:18

Translating 31P NMR references

2 1 7k
answers votes views
Hello, I am trying to compare different 31P chemical shift data from a variety of literature sources. However, each of...
Posted: David Condon 11 / Updated: sekhar Talluri 621 Apr 06 '13 at 11:45

capacity to run 19F13C NMR exp.

5 0 5k
answers votes views
Hi all:) I apologize for my simple ( or well, stupid) question: I was wondering if it is possible to run a 2D 13C19F exp...
Posted: Ala M. 1 / Updated: VVK 61 Nov 27 '12 at 12:24

Varian VNMRJ 2.2D standard experiments

3 1 7k
answers votes views
Hey folks, I was wondering if anyone else has had any issues with the experiments/pulse sequences that came standard in ...
Posted: CaymanDave 11 / Updated: Bharathwaj 112 Nov 09 '12 at 08:16

Long-range 1H-{15N} coupling (HMBC)

3 1 8k
answers votes views
Hello All I have two examples of what appears to be 6J coupling between 1H and 15N using gradient HMBC experiments with...
Posted: Craig 41 / Updated: Evgeny Fadeev 5771 Feb 16 '13 at 15:17

Processing 3QMAS using nuts

0 0 1k
answers votes views
Does anyone know how to shearing 3QMAS 2D spectra using nuts? Or is there a macro for 3QMAS in nuts? ...
Posted: Jun Xu 1 Oct 24 '12 at 18:30

2D NOESY filtered/edited experiments

3 0 6k
answers votes views
what are the name for 2D NOESY filtered/edited experiments like F1fF2f, F1fF2e, F2f, F1eF2e in Bruker 700MHz ?. Here is ...
Posted: ambrish 1 / Updated: marcs 11 Nov 28 '12 at 08:13

File with description of parameters

1 0 4k
answers votes views
Hi, comrades! We use the linux version of TopSpin 2.1 as a software for AVANCE II Bruker 400 MHz. As I found parameters ...
Updated: Alexander Abakumov 11 Nov 19 '12 at 09:16

Pulse sequence for homonuclear correlation with SPC5 mixing period

0 0 9k
answers votes views
Hi, I'm looking for a pulse program suitable for Bruker Avance llI (topspin 2.1) which is 2D homonuclear correlation wit...
Posted: Gili Abr 1 Sep 27 '12 at 02:09

Amplifier Error

1 0 4k
answers votes views
Hi All, I am worikng with 500Mhz Bruker. Recently 3rd channel is installed in my institute when i run 3D HNCA experime...
Posted: saship21 21 / Updated: sekhar Talluri 621 Oct 21 '12 at 07:05

Setting up deuterium (2H) NMR on Bruker BBO probe

2 0 12499
answers votes views
I need to set up 2H NMR on a Bruker 600 MHz BBO probe for the first time. Can anyone give me a set of instructions? Do ...
Posted: doodlebugs 1 / Updated: Anne Baudouin 36 Oct 15 '12 at 02:37

window resizing issues with nmrDraw on Ubuntu 12.04

0 0 2k
answers votes views
Hi all, I upgraded to Ubuntu 12.04 and reinstalled nmrpipe. Everything works well - except that when I resize the nmrDr...
Posted: Kaustubh 21 Sep 15 '12 at 18:33

qfil: baseline correction

3 0 5k
answers votes views
"the t1 dimensio was processed using the baseline correction mode “qfil” in the XWINNMR software" Does anyone know abou...
Posted: Frey 61 / Updated: Kirk Marat 711 Sep 19 '12 at 13:11

Software for 2D Peak Deconvolution/Fitting

2 1 5k
answers votes views
Hello all, I have 1H-15N HSQC spectra that contain pairs of partially overlapped crosspeaks for which I need to extract...
Posted: ChemMJW 99 / Updated: delaglio 1361 Sep 14 '12 at 09:34

sensitivity in bruker 400 mHz NMR

1 0 5k
answers votes views
hiii everyone, our receiver gain is half of normal.(normal mean according to training report is 1024 but when we make a...
Posted: b t 1 / Updated: Kirk Marat 711 Aug 31 '12 at 07:32

How much Cr(acac) for quant carbons

1 0 11944
answers votes views
What's the standard amount or concentration for using Cr(acac) in a Quantitative Carbon? I read 30mg per ml but that is...
Posted: Javaslinger 53 / Updated: Pascal Fricke 236 Aug 18 '12 at 10:17

multiple-quantum diffusion measurements

1 1 3k
answers votes views
Dear all, My first question here, I hope that someone can help! In a paper describing a method to measure diffusion us...
Updated: Daan 11 Sep 11 '12 at 00:34

topshim error

2 0 9k
answers votes views
Hi everyone,I have problem on Bruker AV-500 in runing topshim. when I run topshim it give error that during raw check z ...
Posted: feroon 21 / Updated: jen 1 Nov 07 '13 at 04:29

Longer pulse length for conducting samples?

3 0 6k
answers votes views
Hi, my 1H 90 deg pulses are always longer when I work with my samples that are electrically conducting. Is this a/the re...
Posted: Santhosh 1 / Updated: glass lizard 31 Aug 12 '12 at 12:24

Chemical shift evolution compensation for delays flanked by hard or soft pulses

0 1 2k
answers votes views
Hello all, In a typical 2D experiment, the delay "d0" (t1 increment delay in Bruker) is compensated for the chemical sh...
Posted: Jana 31 Aug 02 '12 at 10:33

Observing protein-ligand interaction using weakly soluble compounds

1 0 9k
answers votes views
I am trying to setup an experiment using HSQC with titration of a compound to determine the Kd of the protein-ligand int...
Posted: Patrick Bartlow 1 / Updated: Justin Frank 1 Jun 29 '16 at 19:39

Does anybody know techmag spectrometers?

1 0 3k
answers votes views
Hi, Techmag (http://www.tecmag.com/index.html) argues to have quite good spectrometers (redstone, discovery 3, scout .....
Posted: fid 139 / Updated: Jeffreyyyy 1 Feb 27 '15 at 12:32

Spectral Binning

0 0 2k
answers votes views
Hey all-- I've noticed that many metabolomics papers use principal components analysis of the data, involving spectral ...
Posted: DVigneault 3 Jul 23 '12 at 13:47

centrifuge or porous-plate

0 0 1k
answers votes views
centrifuge or porous-plate; which of these techniques is better for estimation of T2 cut-off in NMR core measurements ...
Posted: jk 1 Jul 19 '12 at 03:03

How long is too long a pulse?

3 0 4k
answers votes views
I recently calibrated the 90 for 11B on a Agilent 400MR. The 1H 90 was about 6.8us at 59dB... It turns out to be 22.6u...
Posted: Javaslinger 53 / Updated: Thomas barbara 11 Oct 16 '12 at 15:36

How to set up an instrument for 11B NMR?

1 0 4k
answers votes views
Ok, on this particular Agilent MR400 we have a OneProbe with a ProTune module. It's presently set up and calibrated for...
Posted: Javaslinger 53 / Updated: jkurutz 131 Jul 19 '12 at 05:54
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