Tagged questions

c13 edit noesy phasing problem

3 1 4k
answers votes views
Dear NMR Wiki'ers I Have acquired c13-edit noesy experiment on 700vnmr for my target protein (20kd) with mixing time of...
Justin Frank 1 Jun 29 '16 at 19:48

Beginner: Help understanding 3D NOESY

2 0 4k
answers votes views
Hi, I am a person with a Computer Science background who is dealing with some NMR dataset. I am concerned with the assig...
Justin Frank 1 Jun 27 '16 at 23:13

N15 NNH separated NOESY

2 0 3k
answers votes views
Dear friends, I came across one reference which describe N15 NNH separated NOESY experiment but I am not able to get ...
Justin Frank 1 Jun 27 '16 at 22:53

simulate 3D NOESY

1 0 3k
answers votes views
Hello everyone I am using NMRPipe to simulate 3D, noise-free NOESY spectra (13C or 15N resolved) from peak lists, but I...
canavanin 17 Mar 19 '14 at 05:17

1D NOESY with selective excitation and water suppression

2 0 9k
answers votes views
Hi, I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow ...
kyleabeauchamp 1 Mar 06 '14 at 15:37

selective noesy

5 0 3k
answers votes views
Hi, I have question for you who have Bruker instruments! On 300 MHz I worked on selective noesy pulse sequence (selnogp)...
Pete Gierth 401 Feb 13 '14 at 14:58

How to start experiment at particular row

1 0 2k
answers votes views
I was running a 3D 15N edit NOESY (on a Bruker 600) and it was inadvertently stopped. I would like to restart the experi...
Pete Gierth 401 Jun 13 '13 at 11:05

Difference between 13C edited and 13C resolved NOESY

1 0 3k
answers votes views
Hii, I want to know the difference between 13C edited and 13C resolved NOESY and also tell me about the applicati...
sekhar Talluri 621 May 25 '13 at 12:30

Calculating NOESY intensities from X-ray crystal structure

0 0 1k
answers votes views
Hi folks, Has anyone calculated NOESY intensities from a X-ray crystal structure? CORMA program calculates. But have an...
nmrspin 1 May 10 '13 at 11:39

Why do we turn off sample spinning when we do NOESY experiment?

2 1 4k
answers votes views
Why do we trun off sample spinning when we do NOESY experiment? ...
Evgeny Fadeev 5771 Jun 03 '12 at 17:23

"quiet" NOESY

1 0 3k
answers votes views
How to set "quiet" NOESY experiment on spectrometer Bruker ? ...
David O'Brien 1 Apr 25 '12 at 08:38

Isotope Filtered NOESY

1 0 6k
answers votes views
I'm an NMR newbie. I know isotope filtering can be used to study a complex under slow exchange, but for fast-exchange, i...
Eugene DeRose 41 Feb 28 '12 at 12:56

Folding vs. Aliasing (in NOESY spectra)

1 0 6k
answers votes views
Hi everyone Does anyone happen to know if/how I can tell whether peaks outside the spectral width were either folded or...
Kirk Marat 711 Feb 14 '12 at 13:38

Problem while genrating the constraints form N-15 edit AND C-13 EDIT NOESY files

1 0 2k
answers votes views
I am encountering the problem while generating the constraints form N-15 edit AND C-13 EDIT NOESY files that very few c...
sekhar Talluri 621 Jan 15 '12 at 11:19

Auto assignment of N15 C13 EDIT noesy experments

1 0 3k
answers votes views
Dear NMR wikiers could you please suggest any auto assignment web servers online to assign N-15 & C13- EDIT NOESY...
sri 71 Oct 17 '11 at 10:20

NOESY+WET 2D artifacts

0 0 2k
answers votes views
When i run 2d noesy with wet suppression (Hypercomplex with phase=1,2, nt=32, so phasecycle errors should be excluded)),...
Nikolay Boev 41 Sep 07 '11 at 06:07

c13 filter noesy experment for homodimer protein

1 0 3k
answers votes views
Dear NMR wikiers I would like to take c13 filter noesy experment ( this experment explore residues of the interface of...
sri 71 Jul 05 '11 at 10:56

NOESY peak signs

3 1 6k
answers votes views
I am new to NOESY, so I have a number of question about interpreting resulting 2D spectra. Mixing time is 300 ms, substa...
Scott Robson 156 Nov 02 '10 at 10:33

Varian Biopack pulse sequences for intermolecular NOEs

1 1 4k
answers votes views
Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the mom...
Evgeny Fadeev 5771 Aug 10 '10 at 16:52

What is the more effective method of water suppression for NOESY?

2 0 9k
answers votes views
Hi I've been reading about WET water suppression scheme here where it is mentioned as superior to WATERGATE in that it a...
georgeg 301 Jul 19 '10 at 08:53

Will intermediate exchange give poor half filtered NOE

1 1 5k
answers votes views
I have recorded 2D half filtered NOE experiments on a protein-protein complex and got very poor quality data, why would ...
Evgeny Fadeev 5771 Jul 15 '10 at 08:17

How should molecule flexibility affect NOE distance restraint calibration?

1 0 6k
answers votes views
How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it i...
Evgeny Fadeev 5771 Mar 13 '10 at 18:48

What software can do relaxation matrix analysis of NOE?

1 0 5k
answers votes views
Hello, could you suggest software to conduct proper relaxation matrix analysis of NOE? Is it available anywhere on the ...
sekhar Talluri 621 Feb 13 '10 at 12:43

How to set NOESY mixing time in intermediate tumbling regime

1 0 7k
answers votes views
I need to run noesy for a monomeric peptide of about 1.5kDa at 800 MHz 1H frequency. NOE peaks are weak at 100 ms mixing...
sekhar Talluri 621 Feb 13 '10 at 12:00

Select a reasonable NOESY mixing time given MW, B, viscosity?

2 1 7k
answers votes views
Is there a formula that would suggest a reasonable starting value for the mixing time based on the molecular weight, mag...
Evgeny Fadeev 5771 Feb 12 '10 at 12:32
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