i like this post (click again to cancel)
1
i dont like this post (click again to cancel) remove favorite mark from this question (click again to restore mark)

I am new to NOESY, so I have a number of question about interpreting resulting 2D spectra. Mixing time is 300 ms, substance - lactic acid. I have a NOESY spectrum (pulse program - noesygphp) with several peaks having sign structure as:

 -
-+-
 -

I know that the peak with the sign "-" corresponds to dipolar-dipolar interaction, "+" - to chemical exchange. But I don't understand what my result means.

asked Nov 01 '10 at 22:30

Dmitry%20Mainichev's gravatar image

Dmitry Mainichev
31

Hey, maybe you can post up image of the spectrum? Just edit your question and hit the "image" button. - Evgeny Fadeev (Nov 02 '10 at 09:11)


3 Answers:
i like this answer (click again to cancel)
1
i dont like this answer (click again to cancel)

If those peaks are correlations between J-coupled protons, then such shape is likely caused by the coupling, but data truncation as Dan mentioned can explain such pattern too.

Take a look at chapter "Complications with NOESY" in the book "High-Resolution NMR Techniques in Organic Chemistry" by Tim Claridge.

In lactic acid there is no room for chemical exchange aside from hopping of hydroxyl protons to/from water, which probably happens too fast for NMR to capture.

Also, the sign of NOE peak depends on the rate of tumbling of the molecule, which depends on the size of the molecule, viscosity of the solvent and temperature. In small molecules that tumble fast NOE peaks will have sign opposite to the diagonal (so in lactic acid NOE peaks are negative). In larger molecules NOE's will have the same sign as the diagonal peaks. This is because there are two "components" responsible for the emergence of the NOE peaks that make contributions of opposing signs (called zero and double quantum cross-relaxation). Sign of the peak will depend on which component is more significant. Take a look in some NMR text.

link

answered Nov 02 '10 at 09:48

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

updated Nov 02 '10 at 10:30

i like this answer (click again to cancel)
0
i dont like this answer (click again to cancel)

Hi Dmitry, we can't really help without an image - but what your describing sounds a lot like truncation artifacts:

Certain NOESY signals can be very strong, and without appropriate windowing can take a 'sinc-wriggle' shape. This is often corrected for by using an exponential windowing function or similar before the Fourier transform.

link

answered Nov 02 '10 at 09:23

DanODonovan's gravatar image

DanODonovan
21

i like this answer (click again to cancel)
0
i dont like this answer (click again to cancel)

Looks like sinc wiggles in both dimensions due to truncation of the data which may be caused by having a strong signal. This could be fixed when processing the data by introducing window functions that make the signal decay to zero (artificially) before the end of collection of the data. Hard to tell without a real figure to go off.

link

answered Nov 02 '10 at 10:33

Scott%20Robson's gravatar image

Scott Robson
156

Your answer
Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)
toggle preview

powered by CNPROG