Tagged questions

Select a reasonable NOESY mixing time given MW, B, viscosity?

2 1 6k
answers votes views
Is there a formula that would suggest a reasonable starting value for the mixing time based on the molecular weight, mag...
Updated: Evgeny Fadeev 5771 Feb 12 '10 at 12:32

Will intermediate exchange give poor half filtered NOE

1 1 4k
answers votes views
I have recorded 2D half filtered NOE experiments on a protein-protein complex and got very poor quality data, why would ...
Posted: TomgA20 61 / Updated: Evgeny Fadeev 5771 Jul 15 '10 at 08:17

Varian Biopack pulse sequences for intermolecular NOEs

1 1 4k
answers votes views
Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the mom...
Posted: acalab 21 / Updated: Evgeny Fadeev 5771 Aug 10 '10 at 16:52

NOESY peak signs

3 1 5k
answers votes views
I am new to NOESY, so I have a number of question about interpreting resulting 2D spectra. Mixing time is 300 ms, substa...
Posted: Dmitry Mainichev 31 / Updated: Scott Robson 156 Nov 02 '10 at 10:33

c13 edit noesy phasing problem

3 1 3k
answers votes views
Dear NMR Wiki'ers I Have acquired c13-edit noesy experiment on 700vnmr for my target protein (20kd) with mixing time of...
Posted: sri 71 / Updated: Justin Frank 1 Jun 29 '16 at 19:48

Why do we turn off sample spinning when we do NOESY experiment?

2 1 4k
answers votes views
Why do we trun off sample spinning when we do NOESY experiment? ...
Posted: Kiu Chor Sham 11 / Updated: Evgeny Fadeev 5771 Jun 03 '12 at 17:23

How to set NOESY mixing time in intermediate tumbling regime

1 0 6k
answers votes views
I need to run noesy for a monomeric peptide of about 1.5kDa at 800 MHz 1H frequency. NOE peaks are weak at 100 ms mixing...
Posted: Evgeny Fadeev 5771 / Updated: sekhar Talluri 621 Feb 13 '10 at 12:00

What software can do relaxation matrix analysis of NOE?

1 0 5k
answers votes views
Hello, could you suggest software to conduct proper relaxation matrix analysis of NOE? Is it available anywhere on the ...
Posted: Evgeny Fadeev 5771 / Updated: sekhar Talluri 621 Feb 13 '10 at 12:43

How should molecule flexibility affect NOE distance restraint calibration?

1 0 6k
answers votes views
How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it i...
Updated: Evgeny Fadeev 5771 Mar 13 '10 at 18:48

What is the more effective method of water suppression for NOESY?

2 0 9k
answers votes views
Hi I've been reading about WET water suppression scheme here where it is mentioned as superior to WATERGATE in that it a...
Posted: Evgeny Fadeev 5771 / Updated: georgeg 301 Jul 19 '10 at 08:53
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