Question

Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the moment from looking at the Biopack library, I have found 4 pulse sequences which will do this and each provide different information (gnoesyNhsqc_CN, gnoesyNhsqc_NN, gnoesyChsqc_CC and gnoesyChsqc_NC). Would people recommend using a combination of all four of these experiments to determine the intermolecular NOEs for use in structure calculations?

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Hmm, this is really odd, but in our deployment of BioPack the sequences you are mentioning have been replaced with the following statement (or similar):

/*  gnoesyChsqc_NC   for Heterodimer Studies
    has been temporarily removed
    for pulse sequence revision
*/

I've found the following isotope-filtered sequences in the library:

CNfiltocsy.c          gnoesyChsqc_CNfilt.c
gChmqcnoesy_CNfilt.c  gnoesyNhsqc_CNfilt.c

Also, according to the Varian/Agilent user library (you'll have to find the biopack page there and register first) the noesy-hsqc sequences were updated in Dec 2009.

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answered Aug 10 '10 at 14:45

Evgeny Fadeev
577●1

updated Aug 10 '10 at 16:52

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