Unanswered questions

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

2 1 13674
answers votes views
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Justin Frank 1 Jun 27 '16 at 19:27

Protein-peptide binding NMR Experments

2 0 8k
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Hello friends , my target Protein is existing as multimer (i.e Monomer,dimer,tetrameter ) before binding to the peptid...
Justin Frank 1 Jun 27 '16 at 03:02

Difference between H 50 W and X 100 W Amplifiers

2 0 4k
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We have been trying to get a 2D H-F experiment to work using the X 100 W amplifier on our Bruker NMR, but can't get it t...
Kirk Marat 711 Jun 14 '16 at 11:55

When to use 'BLKGRAD' and 'UNBLKGRAD' in a bruker pulse sequence ?

1 0 7k
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I'm trying to modify some pulse sequence on bruker 400, and is confused with the use of 'blkgrad' and 'unblkgrad'; and ,...
Kirk Marat 711 Jun 14 '16 at 11:44

method validation in nmr

1 0 3k
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please tell me whether there are method validation in nmr ...
Ulrich Haunz 210 May 04 '16 at 01:31

Shimming for liquid stateNMR

1 0 2k
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Dear All, What should be the intesnities of spinning sides bands in liquid state NMR?. THank YOu ...
Clemens Anklin 416 Nov 27 '15 at 11:26

reversed NOESY/EXSY peaks

1 0 3k
answers votes views
We have a case of a molecule that has two slowly exchanging conformations. In the NOESY experiment we see cross-relaxing...
Pete Gierth 401 Nov 23 '15 at 11:26

1H 1D NMR data Normalization in Topspin.

1 0 4k
answers votes views
I have performed a series of 1H 1D experiments with different concentration of peptide and accordingly different numbers...
Pete Gierth 401 Nov 23 '15 at 11:14

incorporating fluorine into proteins

2 0 4k
answers votes views
Hi I was considering the possibility of trying some fluorine NMR and was wanting to know if anyone knew any simple ways...
sdc 1 Nov 13 '15 at 13:33

Amplitude problems when comparing 'popt' experiments in Bruker (Topspin)

0 0 9k
answers votes views
Hi all, My question is about the Multiple Display (.md) function on TopSpin (Bruker AVIII). I did some 'popt' ( paramete...
TiagoB1 1 Nov 11 '15 at 03:40
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