2D ROESY-NMR with ~0 peak intensity

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I have been trying to elucidate the 3D structure of oxidized glutathione (M.Wt= 612.63 g/mol) with the help of roesy distance restrains. However, the intensity of roesy peaks is coming out to be 0. Instrument: Bruker Avance II (2005 model) spectrometer. operating frequency: 400 MHz Molar concentration of GSSG= 200mM, pH=7.4 Mixing time= 600msec Relaxation delay= 3 Sec processor= ACD/NMR processor Please give some suggestions to avail appropriate peak intensity.

roesy

asked Jan 18 '14 at 02:50

Raj
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Well, first I will process the spectrum using Bruker software to check if you have similar results. Then I will reduce the spin lock to 300 ms. Finally, not related answer, I think GSSG have no structure. GSH alone is unstructured in solution, there are several evidences in literature. Check if you have aggregation, GSH aggregates at 150 mM. Good luck

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answered Jan 20 '14 at 22:46

marcs
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Asked: Jan 18 '14 at 02:50

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Last updated: Jan 20 '14 at 22:46

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