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hello, I am trying to identify and quantify of metabolites present in semen with spectra obtained from 400 MHz NMR using chenomx software. I have fit about 43 metabolites. I am sure that we must have ATP in our samples but it can not be fit and clusters in 6.1, 8.3 and 8.5 have been missed. So I am not sure about my results and I don't know what has happened. thank you for your answer.

asked Jan 07 '14 at 07:01

Reza%20mohammadi's gravatar image

Reza mohammadi

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Dear Reza,

What was your acquisition software of the spectrometer? What are your concentrations of your complex with ATP in mols? Do not calculate in the stabilisation with salts!

Do you talk about 31P nmr of ATP? Sometimes, when the concentration is low, you have to collect more scans, until you see the typical three ATP signals! Do you tune and match on 31P properly also this is useful on 1H!

Which spectrometer do you use? Bruker/Jeol/Varian/Agilent???

Chenomx is a software for data manipulation, not for acquisition.




answered Feb 10 '14 at 01:36

Ulrich%20Haunz's gravatar image

Ulrich Haunz

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