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Dear NMRWikiers, I'm fresh in nmr field and would like to ask couple questions:
1. Our protein has about 132 residues.Th...
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I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the fol...
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Instrument is a Varian and it would be a long term consultancy/contract. Possibility for direct Hire.
Please direct res...
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Hi everybody,
Has anyone ever seen a C-6 at 64 ppm in the 13C NMR spectrum of a sulfated galactose?
...
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Hi all,
I've been asked to give a "wet lab" tutorial on NMR -- the audience is a mixture of chemists, biologists, and b...
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I am encountering the problem while generating the constraints form N-15 edit AND C-13 EDIT NOESY files that very few c...
| 2 |
1 |
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votes |
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Is liquid water's relaxation rate strongly dependent on temperature, and does anyone have a link to a good online articl...
| 1 |
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Dear friends,
I am in process of doing structure calculation of a dimeric protein. The problem I faced is regarding the...
| 2 |
1 |
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| answers |
votes |
views |
I have a small distortion in the HOD peak in my 1D DOSY spectra. However, it only occurs for the HOD signal and no other...
Updated:
cyg
36
Jan 03 '12 at 21:45
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1 |
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Hello all nmr wikier last week we have upgraded software topspin2.1 to topspin3 on bruker av400 machine.when we use halt...
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And how to you match it to the NMR coil?
Basically I'm looking to perform NMR experiments with frequencies in the 500KH...
| 2 |
0 |
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votes |
views |
I'm looking for a good kinetics experiment to run in the lab section of our graduate lab course. It should be a reaction...
| 2 |
0 |
3k |
| answers |
votes |
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a query again?
I recorded an DQF on deuterated solid sample. The basic pulse programme taken from bruker, optimised the...
| 0 |
0 |
9k |
| answers |
votes |
views |
Can somebody help me with the one query?
How to record an E.COSY for solids proteins?
Its possible to use available prog...
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1 |
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| answers |
votes |
views |
Dear colleagues!
13C-1H J-couplings are essential in some areas of molecule structure research.
I discovered modern met...
Updated:
VVK
61
Nov 29 '11 at 12:37
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Can somebody please send the EBURP-2 wavelib file for VNMR?
Thanks!
AL
...
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1 |
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Dear NMR Wiki'ers
I Have acquired c13-edit noesy experiment on 700vnmr for my target protein (20kd) with mixing time of...
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9k |
| answers |
votes |
views |
Is it possible to generate a spectrum from BMRB chemical shifts?
I want to generate a spectrum to use as a reference fo...
| 2 |
0 |
4k |
| answers |
votes |
views |
I have recently attempted to run a 1H-15N heteronuclear NOE experiment in vnmrj (700 rt probe) using the standard sequen...
| 2 |
0 |
8k |
| answers |
votes |
views |
Hello friends ,
my target Protein is existing as multimer (i.e Monomer,dimer,tetrameter ) before binding to the peptid...
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0 |
1k |
| answers |
votes |
views |
Trying to do molecular dynamics with explicit water molecules I came across a problem with SHAKE routine:
shake
...
Posted:
zltn
1
Oct 27 '11 at 13:51
| 2 |
0 |
4k |
| answers |
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Could you please describe about advantages of CYANA2.1 and ARIA1.2 software to calculate the structure of protein ? w...
Updated:
sri
71
Oct 28 '11 at 03:01
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0 |
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to detect short T2 NMR relaxation time, we use high gradient strengths. but how? what is the relation? what is the mecha...
| 3 |
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| answers |
votes |
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I have pulse programs which work great on our varian INOVA instruments, but when I transfer them to varian VNMRS I get e...
| 1 |
1 |
4k |
| answers |
votes |
views |
When I try to launch the spincad utility in the command line of VNMRJ v. 2.2c I get the following error:
Error in start...
Updated:
dkubicki
11
Nov 01 '11 at 11:26
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1 |
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Hi I am observing some unexpected cross peaks in some homonuclear protein data and I was wanting to know if anyone had a...
| 4 |
1 |
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| answers |
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Hi guys, sorry to be really cheeky and sign up simply to ask for help, but here goes.
I've done a simple Grignard addit...
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| answers |
votes |
views |
Dear Friends,
I've recently encountered a problem with gradient shimming that has never happened to me before. I can't ...
| 2 |
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| answers |
votes |
views |
Dear Friends,
I am not able to figure out how to determine the side chain assignment of Last C-terminal SERINE residue...
| 0 |
0 |
5k |
| answers |
votes |
views |
hello nmr wikier I am using Z-gradiant BBI probe on bruker AV-500 since last weak I am facing tuning and matching proble...
Posted:
feroon
21
Oct 05 '11 at 08:16
