Tagged questions

When to label peptides and proteins and when not?

1 0 87
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When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with: 15N 13C both ...
Updated: Evgeny Fadeev 2571 6 hours ago

2D pulse sequences for NMR of protein/peptide complexes?

0 1 172
answers votes views
Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone ...
Posted: acalab 11 / Updated: Evgeny Fadeev 2571 Jul 26 at 09:25

Eliminating NOE restraints

1 1 257
answers votes views
I have collected over 370 NOE restraints for a 38 AA peptide. Because I have less than 10 restraints per residue, I am ...
Posted: E V Clarke 11 / Updated: sekhar Talluri 301 May 23 at 12:18

Software to model a 9-mer peptide that binds divalent metals and may oligomerize?

1 1 333
answers votes views
I'm gearing up for a project in which we're looking at the structure of a short peptide that should fold upon binding di...
Posted: jkurutz 71 / Updated: Evgeny Fadeev 2571 Mar 18 at 09:32

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

1 0 455
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By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry. Also - ...
Updated: Evgeny Fadeev 2571 Feb 27 at 10:23

finding sample data for practicing 2D 1H assignment

2 1 1k
answers votes views
as I have already started to assign a small peptide, I need sample data set for practicing 2d h1 assignment, could you p...
Posted: niloofar niknam 26 / Updated: jkurutz 71 Dec 15 '09 at 05:00
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