finding sample data for practicing 2D 1H assignment

1	as I have already started to assign a small peptide, I need sample data set for practicing 2d h1 assignment, could you please guide me how I can find one? resonance-assignment peptide learning asked Aug 31 '09 at 17:46 niloofar niknam 26 updated Nov 18 '09 at 14:27 Evgeny Fadeev 54●1

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I just taught a grad student lab course where we assigned bacitracin, a nonribosomal 11-mer cyclic peptide with a couple of ornithines and D-amino acids. I'll be happy to share the raw data.

link

answered Dec 15 '09 at 05:00

jkurutz
21

Hi Josh, you can upload file here - http://nmrwiki.org/wiki/index.php?title=Special:Upload (copy-paste in your browser) and then add the link to your answer - Evgeny Fadeev (Dec 15 '09 at 09:35)

BMRB data bank has a number of raw data sets with assignments.

Here is one record for a 19 residue peptide, and the spectra are here. There are a couple of other peptide data sets.

Also a book "NMR of proteins and nucleic acids" by Kurt Wuthrich may be helpful. There are four chapters on protein assignments.

cheers.

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answered Sep 01 '09 at 10:31

Evgeny Fadeev
54●1

updated Sep 02 '09 at 13:06

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Asked: Aug 31 '09 at 17:46

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Last updated: Dec 15 '09 at 05:00

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