Tagged questions

!3C NMR of Peptide

0 0 330
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Hi, thank you for reading my question. I am a novice at NMR, I apologize in advance if my question appears naive. I am ...
Posted: pjb 1 Jun 11 '13 at 00:32

Low resolution of pepitde samples

3 0 1k
answers votes views
Dear friends, I am now trying to record the NOESY spectrum of my 30 amino acids peptide on DRX600 with a TXI cryo probe...
Posted: Yun Wang 1 / Updated: Evgeny Fadeev 5471 Jun 07 '11 at 14:00

peptide protein interaction

5 1 2k
answers votes views
I have determined the structure of a 110 residues protein(11kDa) which is known to interact with a 15mer peptide. Now I ...
Posted: man05 11 / Updated: SankarampadiAravamudhan 91 Mar 06 '11 at 09:06

When to label peptides and proteins and when not?

1 0 2k
answers votes views
When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with: 15N 13C both ...
Posted: Evgeny Fadeev 5471 / Updated: Paul Driscoll 81 Feb 02 '11 at 04:28

2D pulse sequences for NMR of protein/peptide complexes?

0 1 1k
answers votes views
Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone ...
Posted: acalab 21 / Updated: Evgeny Fadeev 5471 Jul 26 '10 at 09:25

Eliminating NOE restraints

1 1 1k
answers votes views
I have collected over 370 NOE restraints for a 38 AA peptide. Because I have less than 10 restraints per residue, I am ...
Posted: E V Clarke 11 / Updated: sekhar Talluri 601 May 23 '10 at 12:18

Software to model a 9-mer peptide that binds divalent metals and may oligomerize?

1 1 1k
answers votes views
I'm gearing up for a project in which we're looking at the structure of a short peptide that should fold upon binding di...
Posted: jkurutz 111 / Updated: Evgeny Fadeev 5471 Mar 18 '10 at 09:32

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

2 0 3k
answers votes views
By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry. Also - ...
Posted: Evgeny Fadeev 5471 / Updated: SankarampadiAravamudhan 91 Mar 17 '11 at 01:01

finding sample data for practicing 2D 1H assignment

2 1 2k
answers votes views
as I have already started to assign a small peptide, I need sample data set for practicing 2d h1 assignment, could you p...
Posted: niloofar niknam 26 / Updated: jkurutz 111 Dec 15 '09 at 05:00
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