Tagged questions

When to label peptides and proteins and when not?

1 0 87
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When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with: 15N 13C both ...
Updated: Evgeny Fadeev 2571 6 hours ago

What is your test for the feasibility of protein structure determination?

2 0 96
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How do you evaluate protein samples for the feasibility to solve 3D structure? For example if you record a test HSQC se...
Posted: Evgeny Fadeev 2571 / Updated: Ilango 151 Jul 21 at 12:35

2D pulse sequences for NMR of protein/peptide complexes?

0 1 172
answers votes views
Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone ...
Posted: acalab 11 / Updated: Evgeny Fadeev 2571 Jul 26 at 09:25

What is the minimum information required to calculate a structure with cyana?

1 1 187
answers votes views
Hi guys : I have several spectra, and wanted to test out what data I do , and do not need, in order to effectively get a...
Posted: j 91 / Updated: Bharathwaj 31 Jun 28 at 19:43

How to assign (perturbed) resonances when structure is known?

1 0 280
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I had two problems like this one recently: have protein (in solution) of known general structure resonance assignments...
Posted: Evgeny Fadeev 2571 / Updated: Aleksandr Yakimov 11 Apr 25 at 01:17

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

1 0 342
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I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Updated: Evgeny Fadeev 2571 Mar 08 at 09:45

NMR experiments to assign aromatic sidechains?

2 1 529
answers votes views
Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'...
Posted: anita 31 / Updated: Andrew Fowler 51 Apr 07 at 13:10

NMR of proteins

1 0 517
answers votes views
I need informations about nuclear magnetic resonance of proteins with figures. ...
Posted: f_tahoori 6 / Updated: Evgeny Fadeev 2571 Oct 25 '09 at 11:21

Please suggest model proteins and peptides for NMR

1 1 673
answers votes views
do you know any model proteins except lysozyme suitable for nmr experiments? ...
Posted: niloofar niknam 26 / Updated: Evgeny Fadeev 2571 Jan 23 at 13:19
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