Tagged questions

Buffer systems for protein pH titrations

1 0 936
answers votes views
Hi All, I am trying to design a 1D 31P pH titration experiment for a protein. I have been trying to identify a good buf...
Posted: K Sippel 1 / Updated: ChemMJW 99 Aug 22 at 20:40

How to adjust the receiver gain in a deuterium oxide protein sample?

2 0 1k
answers votes views
I am running a 15N HSQC on a protein sample which is in a 100% deuterium oxide solvent. The command rga in TopSpin gives...
Updated: norsouth 3 Dec 02 '13 at 11:16

Streaks in RDC Gel Spectrum

2 0 1k
answers votes views
Hello all, I have used stretched polyacrylamide gels successfully many times to collect RDC data for proteins. The las...
Updated: ChemMJW 99 Dec 06 '13 at 19:10

1D NOESY with selective excitation and water suppression

2 0 1k
answers votes views
Hi, I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow ...
Posted: slawojek 1 / Updated: kyleabeauchamp 1 Mar 06 at 15:37

csrosetta server application

0 0 385
answers votes views
I upload my protein on csrosetta server for generating structures. I have provided TALOS data file. Its already two days...
Posted: anup 1 Jun 04 '13 at 06:09

selecting for side chains

1 1 802
answers votes views
Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2? ...
Posted: service email start 11 / Updated: Matthew Revington 41 Jun 06 '12 at 10:34

RDC gel preparation

3 0 2k
answers votes views
I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the fol...
Posted: andrewL 1 / Updated: Andrew Fowler 96 Apr 24 '13 at 07:06

Spectra from BMRB Chemical shifts

4 0 2k
answers votes views
Is it possible to generate a spectrum from BMRB chemical shifts? I want to generate a spectrum to use as a reference fo...
Posted: Andreas 1 / Updated: delaglio 1361 Dec 11 '11 at 14:15

Protein-peptide binding NMR Experments

1 0 2k
answers votes views
Hello friends , my target Protein is existing as multimer (i.e Monomer,dimer,tetrameter ) before binding to the peptid...
Updated: sri 71 Oct 31 '11 at 12:20

Line broadening in nmr

5 0 3k
answers votes views
Hello can some body help me in understanding the line broadening effect in NMR? I am working with protein and small mol...
Posted: fatima A 1 / Updated: Evgeny Fadeev 5571 Oct 12 '11 at 07:25
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