i like this post (click again to cancel)
0
i dont like this post (click again to cancel) remove favorite mark from this question (click again to restore mark)

Dear NMR WIKIERS ,

IS THEIR ANY NMR EXPERMENT ON 700 VNMR TO KNOW THE duterium percentage of randomly deuterated protein WHICH PREPARED USING 90 PERCENT D20 AND 10 PERCENT H2O ?

Regards

SRI

asked Jun 21 '11 at 00:10

sri's gravatar image

sri
71

I don't think so. Use mass spectroscopy if you can is the easiest way... - Yoan Monneau (Jun 21 '11 at 01:25)

thank you - sri (Jun 21 '11 at 01:35)


One Answer:
i like this answer (click again to cancel)
2
i dont like this answer (click again to cancel)

I think you are better off forgetting NMR methods for determining the deuteration percentage of your protein. Mass spec (using simple MALDI or ESI) is by far the quickest and easiest method. Simply calculate the expected molecular weight of your protein in a fully-protonated state and in a fully-deuterated state and then apply the following formula:

Average % deuteration = 100 * (experimental MW from mass spec - theoretical fully protonated MW) / (theoretical fully deuterated MW - theoretical fully protonated MW)

link

answered Jun 21 '11 at 10:05

ChemMJW's gravatar image

ChemMJW
99

thank alot . I am new to biomoleculer nmr - sri (Jun 21 '11 at 18:59)

Hi Sri, if you like the answer, please mark it as "accepted" - just click the checkmark below the votes. - Evgeny Fadeev (Jun 21 '11 at 20:32)

Your answer
Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)
toggle preview

powered by CNPROG