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Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'd like to try 2D and 3D.

What's available in the vendor-specific libraries?

Thank you very much.

asked Jan 22 '10 at 16:31

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anita
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updated Jan 26 '10 at 09:58

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Evgeny Fadeev
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There are standard hcch cosy and tocsy experiments: hcchcogp3d, hcchdigp3d, and hcchdigp3d2. And there are the "music" pulse sequences: music_fhyw_3d is for Phe/His/Tyr or Trp. I don't know if there are any other specialized ones.

link

answered Jan 26 '10 at 12:26

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Robert Peterson
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Oh, thank you! Which of those correlate aromatic C and H with CB or HB? - anita (Jan 26 '10 at 16:43)

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Lewis Kay's lab published (HB)CB(CGCD)HD and (HB)CB(CGCDCE)HE experiments quite some time ago. Neither is all that sensitive, but they're only 2D experiments. You can also look for strong intra-residue NOEs between the ring protons and HA/HB, although that requires more caution.

Once you've correlated HD and/or HE to the backbone, you can get the rest of the ring protons with COSY, TOCSY, and NOESY, then the carbons with HSQC/HMQC.

link

answered Apr 07 '10 at 13:10

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Andrew Fowler
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I've done these and they complemented a 13C NOESY quite well. I was able to get a good 80% of my aromatics signals seen by these experiments - roseyp (Feb 22 '11 at 09:38)

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There a number of the C13 detect experiments that might help, the simplest might be the 13C-13C COSY experiment in the aromatic region, see http://onlinelibrary.wiley.com/doi/10.1002/cmr.a.20109/pdf

Of course you have to have access to a carbon detect cryoprobe

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answered Aug 19 '10 at 07:15

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Matthew Revington
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Thank you! We don't have a probe like that yet, unfortunately. - anita (Aug 19 '10 at 10:10)

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