Tagged questions

assignment of 12 kd protein

6 1 5k
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Dear NMR Wikiers ! I encountering problem with assignment of 12 kd protein ! i assigend already 50 percent of backbone ...
Posted: sri 71 / Updated: Thomas Garner 81 Aug 30 '11 at 07:11

Best pulse sequence for HSQC in Guanidine-HCl

3 1 7k
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Hello, I would like to acquire an HSQC spectrum of an 15N-labeled 12 kDa protein unfolding (over the course of hours) i...
Posted: vadim 23 / Updated: Pete Gierth 401 Oct 30 '10 at 06:29

Aromatic sidechain assignment pulse sequences for Varian?

3 0 4k
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Hi, someone asked a similar question before, but I'd like to know: Are there readily available varian pulse sequences t...
Posted: Magnethead 42 / Updated: Dummy 31 Nov 19 '10 at 06:28

When to label peptides and proteins and when not?

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When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with: 15N 13C both ...
Posted: Evgeny Fadeev 5771 / Updated: Paul Driscoll 81 Feb 02 '11 at 04:28

What is your test for the feasibility of protein structure determination?

2 1 4k
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How do you evaluate protein samples for the feasibility to solve 3D structure? For example if you record a test HSQC se...
Posted: Evgeny Fadeev 5771 / Updated: Ilango 171 Jul 21 '10 at 12:35

What is your test for the feasibility of protein structure determination?

2 1 4k
answers votes views
How do you evaluate protein samples for the feasibility to solve 3D structure? For example if you record a test HSQC se...
Posted: Evgeny Fadeev 5771 / Updated: Ilango 171 Jul 21 '10 at 12:35

How to assign (perturbed) resonances when structure is known?

1 0 4k
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I had two problems like this one recently: have protein (in solution) of known general structure resonance assignments...
Posted: Evgeny Fadeev 5771 / Updated: Aleksandr Yakimov 11 Apr 25 '10 at 01:17

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

2 1 10680
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I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Posted: Evgeny Fadeev 5771 / Updated: Justin Frank 1 Jun 27 '16 at 19:27

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

2 1 10680
answers votes views
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Posted: Evgeny Fadeev 5771 / Updated: Justin Frank 1 Jun 27 '16 at 19:27

NMR of proteins

3 0 4k
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I need informations about nuclear magnetic resonance of proteins with figures. ...
Posted: f_tahoori 6 / Updated: Justin Frank 1 Jun 27 '16 at 20:47
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