Tagged questions

NOESY peak signs

3 1 5k
answers votes views
I am new to NOESY, so I have a number of question about interpreting resulting 2D spectra. Mixing time is 300 ms, substa...
Posted: Dmitry Mainichev 31 / Updated: Scott Robson 156 Nov 02 '10 at 10:33

Varian Biopack pulse sequences for intermolecular NOEs

1 1 4k
answers votes views
Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the mom...
Posted: acalab 21 / Updated: Evgeny Fadeev 5771 Aug 10 '10 at 16:52

What is the more effective method of water suppression for NOESY?

2 0 8k
answers votes views
Hi I've been reading about WET water suppression scheme here where it is mentioned as superior to WATERGATE in that it a...
Posted: Evgeny Fadeev 5771 / Updated: georgeg 301 Jul 19 '10 at 08:53

What is the more effective method of water suppression for NOESY?

2 0 8k
answers votes views
Hi I've been reading about WET water suppression scheme here where it is mentioned as superior to WATERGATE in that it a...
Posted: Evgeny Fadeev 5771 / Updated: georgeg 301 Jul 19 '10 at 08:53

Will intermediate exchange give poor half filtered NOE

1 1 4k
answers votes views
I have recorded 2D half filtered NOE experiments on a protein-protein complex and got very poor quality data, why would ...
Posted: TomgA20 61 / Updated: Evgeny Fadeev 5771 Jul 15 '10 at 08:17

How should molecule flexibility affect NOE distance restraint calibration?

1 0 5k
answers votes views
How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it i...
Updated: Evgeny Fadeev 5771 Mar 13 '10 at 18:48

What software can do relaxation matrix analysis of NOE?

1 0 5k
answers votes views
Hello, could you suggest software to conduct proper relaxation matrix analysis of NOE? Is it available anywhere on the ...
Posted: Evgeny Fadeev 5771 / Updated: sekhar Talluri 621 Feb 13 '10 at 12:43

How to set NOESY mixing time in intermediate tumbling regime

1 0 6k
answers votes views
I need to run noesy for a monomeric peptide of about 1.5kDa at 800 MHz 1H frequency. NOE peaks are weak at 100 ms mixing...
Posted: Evgeny Fadeev 5771 / Updated: sekhar Talluri 621 Feb 13 '10 at 12:00

Select a reasonable NOESY mixing time given MW, B, viscosity?

2 1 6k
answers votes views
Is there a formula that would suggest a reasonable starting value for the mixing time based on the molecular weight, mag...
Updated: Evgeny Fadeev 5771 Feb 12 '10 at 12:32

Select a reasonable NOESY mixing time given MW, B, viscosity?

2 1 6k
answers votes views
Is there a formula that would suggest a reasonable starting value for the mixing time based on the molecular weight, mag...
Updated: Evgeny Fadeev 5771 Feb 12 '10 at 12:32
10

questions tagged

noesy

Newest questions are shown first.

posts per page103050

powered by CNPROG