Unanswered questions

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

2 0 19237
answers votes views
By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry. Also - ...
SankarampadiAravamudhan 91 Mar 17 '11 at 01:01

How to study polymer degradation by gamma and X-Ray radiation?

0 1 4k
answers votes views
Hi, I was asked to study polyethylene degradated by gamma and Xrays. I planed to measure relaxations rates, but which on...
Evgeny Fadeev 5771 Mar 16 '11 at 08:10

Why RF irradiation will damage amplifier?

3 1 16736
answers votes views
Here our 1H amplifier of NMR spectrometer is damaged twice for long time or high power RF irradiation. Why the duty cyc...
lbeezley 1 Mar 15 '11 at 08:37

optimising o1 in solid state nmr

1 0 6k
answers votes views
I'm a little bit confused about setting o1, in the case of a solid sample First o1 is the offset of the transmitter fre...
silvernmr 11 Mar 09 '11 at 06:17

transfer of data sets/pulse sequences from Xwinnmr/DRX to Avance III/topspin

1 1 4k
answers votes views
How do I transfer data sets ( with pulse sequences for measurements of various cross- -relaxtion rates) collected unde...
Wendy Gong 1 Mar 09 '11 at 03:31

How to correctly set ROESY spin-lock power?

0 1 6k
answers votes views
I'm using roesyph.2 pulse sequence (Bruker) and I would like to know how to calculate spin-lock power in Hz for this exp...
Evgeny Fadeev 5771 Mar 07 '11 at 22:22

setting up solid echo on bruker

4 1 11189
answers votes views
Hello, I need to set up aquisition parameters for an echo solid sequence on bruker. In order to analyse deuteraed sample...
silvernmr 11 Mar 06 '11 at 12:39

How can I calculate a carbon-proton coupling constant for a molecule?

1 1 8k
answers votes views
I'm trying to explain a missing HMBC peak, and having a coupling constant less than 10 Hz would do that nicely. It's a f...
sekhar Talluri 621 Mar 05 '11 at 09:58

How to pick peaks manually in nmrview?

1 1 5k
answers votes views
Is there any way to pick peaks manually in nmrview using the red/black cursors? ...
Ilango 171 Mar 04 '11 at 09:10

Basics of filtered NOE experiment

1 1 4k
answers votes views
I have not fully understood the mechanism of the filtered NOE experiment. What does it tell you? Are you able to disting...
Evgeny Fadeev 5771 Mar 03 '11 at 17:44

processing varian data in topspin?

4 1 22526
answers votes views
Hi I have recently moved to a lab which has a varian magnet, in the past I have only used brukers. The people here use ...
surya 11 Feb 28 '11 at 06:28

Distinguish between protonation and conformational change?

2 1 5k
answers votes views
Hi all, In a folded protein with 13C-delta labeled glutamates, how can one distinguish between chemical shift due to pr...
SankarampadiAravamudhan 91 Feb 27 '11 at 22:59

Problems about VnmrJ 2.3A(3. fid display style)

0 0 2k
answers votes views
How can I change the line style of the spectrum or fid in viewports? The line of spectrum seems too narrow. ...
Frey 61 Feb 26 '11 at 22:24

Problems about VnmrJ 2.3A (2 shimDriver)

0 0 2k
answers votes views
when shimming with shimDriver, there may be an error message as 'usage - dfid or dfid#' and then the autoshim procedure ...
Frey 61 Feb 26 '11 at 22:23

Problems about VnmrJ 2.3A (1.shimming)

0 1 3k
answers votes views
when shimming with Fid scan, the fid/spectrum can't be displayed in real time. ...
Frey 61 Feb 26 '11 at 22:22

NMR experiments to assign aromatic sidechains?

3 1 9k
answers votes views
Hello, may I ask this - NMR experiments/pulse sequences are available to assign aromatic sidechains - Phe, Tyr, Trp? We'...
roseyp 46 Feb 22 '11 at 09:38

Why is chemical shift range of 13C wider than of 1H nuclei?

6 3 19367
answers votes views
Why the chemical shift range of 1H nmr is ~20 ppm wheras for 13C nmr it is ~230 ppm? ...
Yoan Monneau 136 Feb 15 '11 at 11:23

Question about bandwidth

3 1 8k
answers votes views
Is acquisition bandwidth and receiver bandwidth the same?? am confused with the terminology....please clarify....thanks ...
SankarampadiAravamudhan 91 Feb 14 '11 at 07:36

Pureshift and Pureshift oneshot download problem

2 1 4k
answers votes views
Hi, I am interested in trying these two sequences, I amtrying to work out suitable parameters and thought the "sample da...
David Grace 11 Feb 07 '11 at 02:38

When to label peptides and proteins and when not?

1 0 9k
answers votes views
When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with: 15N 13C both ...
Paul Driscoll 81 Feb 02 '11 at 04:28

Relaxation editing vr paramagnetic relaxation enhancement experiments - 13C CP-MAS NMR

1 1 7k
answers votes views
I am a beginner in NMR spectroscopy and I would like to learn more about relaxation editing experiments vs PRE. A collea...
bmoret42 11 Jan 25 '11 at 12:29

Might anyone donate a lower end NMR instrument?

3 1 6k
answers votes views
Hello, I am working in this really small university and things would be so better if we had a 60 or 90 MHz. I was wonder...
Damodaran Krishnan Achary 21 Jan 21 '11 at 12:51

VNMRJ "No data in data file"

1 1 6k
answers votes views
I'm trying to display a 3D Varian file in VNMRJ. I can see values in the fid file (using the "od" utility) and convert t...
Bharathwaj 112 Jan 19 '11 at 09:05

cs23d vs. cs-rosetta

2 1 6k
answers votes views
Does anybody have any thoughts on this ? I dont really know which is better, CS23D or CS-ROSETTA. Clearly they are ide...
Thomas Garner 81 Jan 19 '11 at 08:05

cs rosetta compilation for linux 32

1 1 5k
answers votes views
Hi guys : I was wondering if anyone knows where I can find CS-Rosetta compiled for 32 bit linux..... Im having trouble ...
Evgeny Fadeev 5771 Jan 13 '11 at 12:27

converting pdb to cyana library file

2 1 6k
answers votes views
hello everyone, i work on modified amino acids. i have cartesian coordinates for these residues from crystal structure...
eiso 31 Jan 13 '11 at 10:03

How to do a Shearing Transform in nmrPipe?

0 1 3k
answers votes views
Hi, In want to do a shearing transform of 2Q-1Q correlation spectra into a 1Q-1Q type projection. This is possible in NM...
Evgeny Fadeev 5771 Jan 05 '11 at 10:18

Where can I find current shim values in Bruker Paravision?

0 1 2k
answers votes views
Hello, I am a novie to the field. I am trying to record the change in settings post shimming using Bruker ParaVision. I...
Evgeny Fadeev 5771 Jan 04 '11 at 15:35

Inverted Spinning Sidebands

2 0 7k
answers votes views
Hello, My 1H MAS NMR spectrum comes out with the right spinning side band pointing up and the left one pointing down. ...
NMRUser61581 13 Jan 03 '11 at 10:04

From what experiment only two bond correlations we can get in varian nmr?

1 1 4k
answers votes views
Hi., i have one compound i need only two bond correlation contours from quaternary carbon to proton. Is there any pulse ...
bernie o'hare 111 Dec 30 '10 at 09:12
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