Unanswered questions

2D NOESY filtered/edited experiments

3 0 6k
answers votes views
what are the name for 2D NOESY filtered/edited experiments like F1fF2f, F1fF2e, F2f, F1eF2e in Bruker 700MHz ?. Here is ...
Posted: ambrish 1 / Updated: marcs 11 Nov 28 '12 at 08:13

File with description of parameters

1 0 4k
answers votes views
Hi, comrades! We use the linux version of TopSpin 2.1 as a software for AVANCE II Bruker 400 MHz. As I found parameters ...
Updated: Alexander Abakumov 11 Nov 19 '12 at 09:16

Pulse sequence for homonuclear correlation with SPC5 mixing period

0 0 9k
answers votes views
Hi, I'm looking for a pulse program suitable for Bruker Avance llI (topspin 2.1) which is 2D homonuclear correlation wit...
Posted: Gili Abr 1 Sep 27 '12 at 02:09

Amplifier Error

1 0 4k
answers votes views
Hi All, I am worikng with 500Mhz Bruker. Recently 3rd channel is installed in my institute when i run 3D HNCA experime...
Posted: saship21 21 / Updated: sekhar Talluri 621 Oct 21 '12 at 07:05

Setting up deuterium (2H) NMR on Bruker BBO probe

2 0 12493
answers votes views
I need to set up 2H NMR on a Bruker 600 MHz BBO probe for the first time. Can anyone give me a set of instructions? Do ...
Posted: doodlebugs 1 / Updated: Anne Baudouin 36 Oct 15 '12 at 02:37

window resizing issues with nmrDraw on Ubuntu 12.04

0 0 2k
answers votes views
Hi all, I upgraded to Ubuntu 12.04 and reinstalled nmrpipe. Everything works well - except that when I resize the nmrDr...
Posted: Kaustubh 21 Sep 15 '12 at 18:33

qfil: baseline correction

3 0 5k
answers votes views
"the t1 dimensio was processed using the baseline correction mode “qfil” in the XWINNMR software" Does anyone know abou...
Posted: Frey 61 / Updated: Kirk Marat 711 Sep 19 '12 at 13:11

Software for 2D Peak Deconvolution/Fitting

2 1 5k
answers votes views
Hello all, I have 1H-15N HSQC spectra that contain pairs of partially overlapped crosspeaks for which I need to extract...
Posted: ChemMJW 99 / Updated: delaglio 1361 Sep 14 '12 at 09:34

sensitivity in bruker 400 mHz NMR

1 0 5k
answers votes views
hiii everyone, our receiver gain is half of normal.(normal mean according to training report is 1024 but when we make a...
Posted: b t 1 / Updated: Kirk Marat 711 Aug 31 '12 at 07:32

How much Cr(acac) for quant carbons

1 0 11944
answers votes views
What's the standard amount or concentration for using Cr(acac) in a Quantitative Carbon? I read 30mg per ml but that is...
Posted: Javaslinger 53 / Updated: Pascal Fricke 236 Aug 18 '12 at 10:17

multiple-quantum diffusion measurements

1 1 3k
answers votes views
Dear all, My first question here, I hope that someone can help! In a paper describing a method to measure diffusion us...
Updated: Daan 11 Sep 11 '12 at 00:34

topshim error

2 0 9k
answers votes views
Hi everyone,I have problem on Bruker AV-500 in runing topshim. when I run topshim it give error that during raw check z ...
Posted: feroon 21 / Updated: jen 1 Nov 07 '13 at 04:29

Longer pulse length for conducting samples?

3 0 6k
answers votes views
Hi, my 1H 90 deg pulses are always longer when I work with my samples that are electrically conducting. Is this a/the re...
Posted: Santhosh 1 / Updated: glass lizard 31 Aug 12 '12 at 12:24

Chemical shift evolution compensation for delays flanked by hard or soft pulses

0 1 2k
answers votes views
Hello all, In a typical 2D experiment, the delay "d0" (t1 increment delay in Bruker) is compensated for the chemical sh...
Posted: Jana 31 Aug 02 '12 at 10:33

Observing protein-ligand interaction using weakly soluble compounds

1 0 9k
answers votes views
I am trying to setup an experiment using HSQC with titration of a compound to determine the Kd of the protein-ligand int...
Posted: Patrick Bartlow 1 / Updated: Justin Frank 1 Jun 29 '16 at 19:39

Does anybody know techmag spectrometers?

1 0 3k
answers votes views
Hi, Techmag (http://www.tecmag.com/index.html) argues to have quite good spectrometers (redstone, discovery 3, scout .....
Posted: fid 139 / Updated: Jeffreyyyy 1 Feb 27 '15 at 12:32

Spectral Binning

0 0 2k
answers votes views
Hey all-- I've noticed that many metabolomics papers use principal components analysis of the data, involving spectral ...
Posted: DVigneault 3 Jul 23 '12 at 13:47

centrifuge or porous-plate

0 0 1k
answers votes views
centrifuge or porous-plate; which of these techniques is better for estimation of T2 cut-off in NMR core measurements ...
Posted: jk 1 Jul 19 '12 at 03:03

How long is too long a pulse?

3 0 4k
answers votes views
I recently calibrated the 90 for 11B on a Agilent 400MR. The 1H 90 was about 6.8us at 59dB... It turns out to be 22.6u...
Posted: Javaslinger 53 / Updated: Thomas barbara 11 Oct 16 '12 at 15:36

How to set up an instrument for 11B NMR?

1 0 4k
answers votes views
Ok, on this particular Agilent MR400 we have a OneProbe with a ProTune module. It's presently set up and calibrated for...
Posted: Javaslinger 53 / Updated: jkurutz 131 Jul 19 '12 at 05:54

Locking in new solvent

2 0 7k
answers votes views
Hello, I am trying to determine solution structure of a peptide in 50% trifluoroethanol(TFE) solution. I dissolved my p...
Posted: GlennG 1 / Updated: JohnW 1 Jul 16 '12 at 15:18

What will happen if I use long pulse?

7 1 12042
answers votes views
Hello everybody. We have an AVANCE 500 MHz bruker instrument. Unfortunately, our amplifier has been weakened and I can n...
Posted: He 21 / Updated: jkurutz 131 Jul 19 '12 at 06:24

Lock Error Bruker Ascend 600

2 0 12902
answers votes views
Hi there, We had a short power failure yesterday and since my boss is in Ireland and our other so called NMR guru does...
Posted: Patrick Seibert 1 / Updated: aaaeee 1 Apr 12 '18 at 12:50

Difficulties with wobbing.

4 1 6k
answers votes views
Hello, dear NMR-comrades! I work on Bruker AVANCE II 400 MHz NMR-spectrometer and today I had some troubles with wobbi...
Posted: Alexander Abakumov 11 / Updated: Jerry Hirschinger 199 Jul 16 '12 at 07:55

Deleting Regions in Sparky

1 0 3k
answers votes views
Hey all-- I'm wondering if there is a way to delete regions of an NMR spectrum in Sparky (or another free software). I...
Updated: DVigneault 3 Jul 05 '12 at 07:21

vertical cylindric oven for NMR tube up to 200C

3 0 3k
answers votes views
Hi I have alraedy seen such a thing, but a google search now ended up with nothing. Does sbdy now where to by such smal...
Posted: fid 139 / Updated: oxy 31 Jul 12 '12 at 03:52

A beginner's guide to protein backbone assignment?

3 0 7k
answers votes views
Hi All, I'm new to protein backbone assignment by NMR, and my protein is about ~37kDa (about 400 residues). We've acqui...
Posted: snoopster398 1 / Updated: Justin Frank 1 Jun 27 '16 at 21:03

How important is calibrating a 90degree pulse for 2D?

2 1 4k
answers votes views
So it is pretty much always recommended to calibrate the 90 for 1H for any 2D experiment... Cosy, Noesy, HSQC, etc... ...
Posted: Javaslinger 53 / Updated: Nikolay Boev 41 Jun 29 '12 at 09:04

Contours in rNMR

1 0 3k
answers votes views
Hey all–– I recently installed the rNMR library (1.1.7) in R (32-bit, 1.40) on my mac (10.6.8). I've been trying to ad...
Updated: DVigneault 3 Jun 29 '12 at 11:00

Karma system blocking people out

1 2 3k
answers votes views
Hi, the karma system is probably a good thing for choosing who can moderate or so. But it is enoying starters a lot. As...
Posted: fid 139 / Updated: Evgeny Fadeev 5771 Jul 12 '12 at 16:04

Lock at high temperatures

2 1 8k
answers votes views
I am having trouble locking a sample of a polymer dissolved in OCDB at 135 degree's. Can this high temperature interfer...
Posted: Javaslinger 53 / Updated: Gareth Morris 71 Jul 08 '12 at 03:02

STDtrNOESY

2 0 3k
answers votes views
Hi Eeveybody, Is there anyone who can help me in processing the STD-Tr-NOESY 2D spectrum, i have done the experiment bu...
Posted: john dawn 1 / Updated: renee 56 Jul 31 '12 at 17:45

1H-119Sn HMBC or HMQC

0 1 1k
answers votes views
Dear experts, I want to run 1H-119Sn HMBC or HMQC, but I don't know the name of pulse program in Bruker. Do you know the...
Posted: Hamidreza Samouei 11 Jun 24 '12 at 03:14

How about calibrating a 13C 90 pulse?

3 0 11478
answers votes views
Most 90 degree pulse calibration instructions I've run across seem to be for a 1H 90. What about a 13C 90 degree pulse?...
Posted: Javaslinger 53 / Updated: Amin os 43 Jun 24 '12 at 05:19

Calibrating 1H decoulpler pulse?

1 0 4k
answers votes views
Recently while getting a loaner probe up and running, I noticed that the engineers simply had me set the decouple 90 to ...
Posted: Javaslinger 53 / Updated: Alexander Marchione 21 Jun 25 '12 at 05:06

Excitation bandwidth for a shaped pulse

3 0 15016
answers votes views
Hi all, I have a small question on excitation bandwidth for shaped pulses. In many of the literature, the field strength...
Posted: Jana 31 / Updated: Pete Gierth 401 Sep 30 '13 at 14:35

ACD/NMR vs. MestReNova calculation difference

2 0 5k
answers votes views
Hi there, how is possible that peak area calculated by ACD/NMR is lower than the same one calculated by MestReNova soft...
Posted: Frantisek Surman 1 / Updated: Kirk Marat 711 Jun 20 '12 at 13:45

t1 Increment delay

1 1 7k
answers votes views
Hello all, iam trying to key in the correct value for nd0 for a custom written pulse program. In doing so i ran into con...
Posted: Jana 31 / Updated: Clemens Anklin 416 Jun 30 '12 at 14:28

eretic standard sample

2 1 6k
answers votes views
I have recently set up the eretic sample concentration measurement experiment experiment on our AvanceIII instrument. ...
Posted: Matthew Revington 41 / Updated: John Hollerton 96 Dec 10 '12 at 08:53

Calibration of a Bruker NMR probe

3 1 7k
answers votes views
I've received a loaner probe from Bruker while ours is being repaired but I'm not getting any timely response on instruc...
Posted: Javaslinger 53 / Updated: Evgeny Fadeev 5771 Jun 21 '12 at 21:03

33S NMR Experiment

1 1 6k
answers votes views
Dear Everyone I´d like to run 33S-NMR experiments using a Bruker NMR spectrometer at 400 MHz buy I nedd the pulse seque...
Posted: Juan_Carlos 11 / Updated: Pete Gierth 401 Jun 15 '12 at 07:53

Gradient strength recalculation

1 0 8k
answers votes views
Iam bit stuck with % z gradient calculation used in Bruker pulse programs. In some instruments (Varian) they use as sho...
Updated: Jana 31 Jun 15 '12 at 09:29

How to process DOSY data by using MestReNova?

3 0 14491
answers votes views
This is my first time in doing DOSY experiment. i got the data but dont know how to process it. ...
Posted: Kiu Chor Sham 11 / Updated: Evgeny Fadeev 5771 Jun 16 '12 at 09:36

Multiple pdb files in pymol

1 0 3k
answers votes views
I have to convert 13000 all atom pdb files into files with c-alpha only. Is there any way i can convert all the files in...
Posted: Tasneem Ali 1 / Updated: renee 56 Jun 13 '12 at 16:03

help on creating a program similar to multizg?

2 1 7k
answers votes views
I am currently trying to write a program that is a lot like the multizg program in the Bruker software, Topspin but with...
Posted: Artjom Nepryahin 11 / Updated: John Hollerton 96 Dec 10 '12 at 10:05

selecting for side chains

2 1 3k
answers votes views
Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2? ...
Posted: service email start 11 / Updated: Justin Frank 1 Jun 29 '16 at 19:22

Unexpected Echo intensity increase in DOSY

2 2 4k
answers votes views
Hi, I ran a typical dosy expt and got an unexpected increase on echo intensity in the begining of the array. The first ...
Posted: fid 139 / Updated: mikaelastewart 11 Jun 03 '13 at 09:14

Why do we turn off sample spinning when we do NOESY experiment?

2 1 6k
answers votes views
Why do we trun off sample spinning when we do NOESY experiment? ...
Posted: Kiu Chor Sham 11 / Updated: Evgeny Fadeev 5771 Jun 03 '12 at 17:23

viewport feature

1 0 3k
answers votes views
The VnmrJ software has a viewport feature. This feature allows the user to view at most 4 spectra (whether 1D or 2D) si...
Posted: addictedtonmr 1 / Updated: Frey 61 May 30 '12 at 17:47

Editing solvent parameters on vnmrj?

1 0 4k
answers votes views
The default parameter setting for Acetic Acid seems to be setting the Lock Power to two. This is a problem when running...
Posted: Javaslinger 53 / Updated: renee 56 May 25 '12 at 15:17
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