protein-protein complex structure determination

i like this post (click again to cancel)

i dont like this post (click again to cancel)

remove favorite mark from this question (click again to restore mark)

Dear Friends,

I am working on a protein-protein complex using 700MHz VARIAN NMR . I have done HSQC perturbation studies by adding incremental ratio of protein A to B, and doing the reverse way adding protein B to A. In both the spectrum I have noticed about 20 peaks disappear or peak intensity drop in both spectrum . The total complex size I assume to be in range of 50kD. Would it be reasonable to proceed further and acquire 3D experiments for protein-protein complex structure calculation ? Any suggestions would be useful.

thanks & regards

Arun

protein-protein complex

asked Mar 14 '12 at 19:50

Arun Gupta
11

One Answer:

oldest newest most voted

0	Are the assignment of resonances for the individual proteins known? If you are sure there is no aggregation, you could try some 3D experiments. Of course, you would want to start with HNCO. link answered Mar 15 '12 at 17:40 service email start 11

Here (once you log in) you will be able to sign up for the periodic email updates about this question.

Your answer

Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)

toggle preview

Tags:

complex ×5
protein-protein ×2

Asked: Mar 14 '12 at 19:50

Seen: 3,851 times

Last updated: Mar 15 '12 at 17:40

Related questions