COSY problem (cosydfesgpph pulse program)

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Hi,

I am running COSY on a DNA:RNA hybrifd. However, I have got no cross-peaks at all even for CYT H5-H6? what do you think the problem is? I am using cosydfesgpph as pulse programme.

Thank you

asked Aug 31 '10 at 02:31

waleed
13

updated Aug 31 '10 at 11:40

Evgeny Fadeev
577●1

no cross-peaks even at lower contour levels? - Evgeny Fadeev (Aug 31 '10 at 11:48)

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Apart from instrumentation setup issues (can't help you there) it is possible that your complex of RNA and DNA is forming a very large particle that relaxes too fast for you to acquire any signal. It's also possible that the DNA/RNA mix is exchanging, broadening your signals too much. Are you positiv your DNA:RNA hybrid forms a tight interaction that does not aggregate?

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answered Aug 31 '10 at 10:32

Scott Robson
156

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bruker ×75
cosy ×3
dna ×2
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Asked: Aug 31 '10 at 02:31

Seen: 4,963 times

Last updated: Aug 31 '10 at 11:48

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