Unanswered questions

Locking in new solvent

2 0 7k
answers votes views
Hello, I am trying to determine solution structure of a peptide in 50% trifluoroethanol(TFE) solution. I dissolved my p...
Posted: GlennG 1 / Updated: JohnW 1 Jul 16 '12 at 15:18

What will happen if I use long pulse?

7 1 11298
answers votes views
Hello everybody. We have an AVANCE 500 MHz bruker instrument. Unfortunately, our amplifier has been weakened and I can n...
Posted: He 21 / Updated: jkurutz 131 Jul 19 '12 at 06:24

Lock Error Bruker Ascend 600

2 0 11373
answers votes views
Hi there, We had a short power failure yesterday and since my boss is in Ireland and our other so called NMR guru does...
Posted: Patrick Seibert 1 / Updated: aaaeee 1 Apr 12 '18 at 12:50

Difficulties with wobbing.

4 1 5k
answers votes views
Hello, dear NMR-comrades! I work on Bruker AVANCE II 400 MHz NMR-spectrometer and today I had some troubles with wobbi...
Posted: Alexander Abakumov 11 / Updated: Jerry Hirschinger 199 Jul 16 '12 at 07:55

Deleting Regions in Sparky

1 0 3k
answers votes views
Hey all-- I'm wondering if there is a way to delete regions of an NMR spectrum in Sparky (or another free software). I...
Updated: DVigneault 3 Jul 05 '12 at 07:21

vertical cylindric oven for NMR tube up to 200C

3 0 3k
answers votes views
Hi I have alraedy seen such a thing, but a google search now ended up with nothing. Does sbdy now where to by such smal...
Posted: fid 139 / Updated: oxy 31 Jul 12 '12 at 03:52

A beginner's guide to protein backbone assignment?

3 0 7k
answers votes views
Hi All, I'm new to protein backbone assignment by NMR, and my protein is about ~37kDa (about 400 residues). We've acqui...
Posted: snoopster398 1 / Updated: Justin Frank 1 Jun 27 '16 at 21:03

How important is calibrating a 90degree pulse for 2D?

2 1 4k
answers votes views
So it is pretty much always recommended to calibrate the 90 for 1H for any 2D experiment... Cosy, Noesy, HSQC, etc... ...
Posted: Javaslinger 53 / Updated: Nikolay Boev 41 Jun 29 '12 at 09:04

Contours in rNMR

1 0 3k
answers votes views
Hey all–– I recently installed the rNMR library (1.1.7) in R (32-bit, 1.40) on my mac (10.6.8). I've been trying to ad...
Updated: DVigneault 3 Jun 29 '12 at 11:00

Karma system blocking people out

1 2 3k
answers votes views
Hi, the karma system is probably a good thing for choosing who can moderate or so. But it is enoying starters a lot. As...
Posted: fid 139 / Updated: Evgeny Fadeev 5771 Jul 12 '12 at 16:04

Lock at high temperatures

2 1 7k
answers votes views
I am having trouble locking a sample of a polymer dissolved in OCDB at 135 degree's. Can this high temperature interfer...
Posted: Javaslinger 53 / Updated: Gareth Morris 71 Jul 08 '12 at 03:02

STDtrNOESY

2 0 3k
answers votes views
Hi Eeveybody, Is there anyone who can help me in processing the STD-Tr-NOESY 2D spectrum, i have done the experiment bu...
Posted: john dawn 1 / Updated: renee 56 Jul 31 '12 at 17:45

1H-119Sn HMBC or HMQC

0 1 1k
answers votes views
Dear experts, I want to run 1H-119Sn HMBC or HMQC, but I don't know the name of pulse program in Bruker. Do you know the...
Posted: Hamidreza Samouei 11 Jun 24 '12 at 03:14

How about calibrating a 13C 90 pulse?

3 0 10881
answers votes views
Most 90 degree pulse calibration instructions I've run across seem to be for a 1H 90. What about a 13C 90 degree pulse?...
Posted: Javaslinger 53 / Updated: Amin os 43 Jun 24 '12 at 05:19

Calibrating 1H decoulpler pulse?

1 0 4k
answers votes views
Recently while getting a loaner probe up and running, I noticed that the engineers simply had me set the decouple 90 to ...
Posted: Javaslinger 53 / Updated: Alexander Marchione 21 Jun 25 '12 at 05:06

Excitation bandwidth for a shaped pulse

3 0 14195
answers votes views
Hi all, I have a small question on excitation bandwidth for shaped pulses. In many of the literature, the field strength...
Posted: Jana 31 / Updated: Pete Gierth 401 Sep 30 '13 at 14:35

ACD/NMR vs. MestReNova calculation difference

2 0 5k
answers votes views
Hi there, how is possible that peak area calculated by ACD/NMR is lower than the same one calculated by MestReNova soft...
Posted: Frantisek Surman 1 / Updated: Kirk Marat 711 Jun 20 '12 at 13:45

t1 Increment delay

1 1 6k
answers votes views
Hello all, iam trying to key in the correct value for nd0 for a custom written pulse program. In doing so i ran into con...
Posted: Jana 31 / Updated: Clemens Anklin 416 Jun 30 '12 at 14:28

eretic standard sample

2 1 6k
answers votes views
I have recently set up the eretic sample concentration measurement experiment experiment on our AvanceIII instrument. ...
Posted: Matthew Revington 41 / Updated: John Hollerton 96 Dec 10 '12 at 08:53

Calibration of a Bruker NMR probe

3 1 7k
answers votes views
I've received a loaner probe from Bruker while ours is being repaired but I'm not getting any timely response on instruc...
Posted: Javaslinger 53 / Updated: Evgeny Fadeev 5771 Jun 21 '12 at 21:03

33S NMR Experiment

1 1 5k
answers votes views
Dear Everyone I´d like to run 33S-NMR experiments using a Bruker NMR spectrometer at 400 MHz buy I nedd the pulse seque...
Posted: Juan_Carlos 11 / Updated: Pete Gierth 401 Jun 15 '12 at 07:53

Gradient strength recalculation

1 0 7k
answers votes views
Iam bit stuck with % z gradient calculation used in Bruker pulse programs. In some instruments (Varian) they use as sho...
Updated: Jana 31 Jun 15 '12 at 09:29

How to process DOSY data by using MestReNova?

3 0 12418
answers votes views
This is my first time in doing DOSY experiment. i got the data but dont know how to process it. ...
Posted: Kiu Chor Sham 11 / Updated: Evgeny Fadeev 5771 Jun 16 '12 at 09:36

Multiple pdb files in pymol

1 0 3k
answers votes views
I have to convert 13000 all atom pdb files into files with c-alpha only. Is there any way i can convert all the files in...
Posted: Tasneem Ali 1 / Updated: renee 56 Jun 13 '12 at 16:03

help on creating a program similar to multizg?

2 1 6k
answers votes views
I am currently trying to write a program that is a lot like the multizg program in the Bruker software, Topspin but with...
Posted: Artjom Nepryahin 11 / Updated: John Hollerton 96 Dec 10 '12 at 10:05

selecting for side chains

2 1 3k
answers votes views
Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2? ...
Posted: service email start 11 / Updated: Justin Frank 1 Jun 29 '16 at 19:22

Unexpected Echo intensity increase in DOSY

2 2 4k
answers votes views
Hi, I ran a typical dosy expt and got an unexpected increase on echo intensity in the begining of the array. The first ...
Posted: fid 139 / Updated: mikaelastewart 11 Jun 03 '13 at 09:14

Why do we turn off sample spinning when we do NOESY experiment?

2 1 6k
answers votes views
Why do we trun off sample spinning when we do NOESY experiment? ...
Posted: Kiu Chor Sham 11 / Updated: Evgeny Fadeev 5771 Jun 03 '12 at 17:23

viewport feature

1 0 3k
answers votes views
The VnmrJ software has a viewport feature. This feature allows the user to view at most 4 spectra (whether 1D or 2D) si...
Posted: addictedtonmr 1 / Updated: Frey 61 May 30 '12 at 17:47

Editing solvent parameters on vnmrj?

1 0 3k
answers votes views
The default parameter setting for Acetic Acid seems to be setting the Lock Power to two. This is a problem when running...
Posted: Javaslinger 53 / Updated: renee 56 May 25 '12 at 15:17
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