Unanswered questions

Total t1 evolution time, TROSY, PRE distance calculation

0 0 3k
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Hi all, This is my first post to the forum and I am admittedly new to the field of protein NMR. I appreciate your patie...
Logan 1 Mar 11 '14 at 09:04

1D NOESY with selective excitation and water suppression

2 0 10442
answers votes views
Hi, I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow ...
kyleabeauchamp 1 Mar 06 '14 at 15:37

How can I tell if and what kind of solvent suppression was used?

2 0 5k
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I have some older 2D HSQC 1H-15N data that was run by a NMR tech. Is there a way I can tell what kind of solvent suppre...
uabnmr1 1 Feb 26 '14 at 07:11

integration 2d noesy

0 0 2k
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I will appreciate if somebody write me how to integrate 2D NOESY spectrum in TopSpin 2.1 or 2.5! Step by step, please! I...
gugica 1 Feb 18 '14 at 03:55

selective noesy

5 0 4k
answers votes views
Hi, I have question for you who have Bruker instruments! On 300 MHz I worked on selective noesy pulse sequence (selnogp)...
Pete Gierth 401 Feb 13 '14 at 14:58

J scaling factor

1 0 4k
answers votes views
Hello How to write a pulse program with J-scaling factor? When I should introduce the scaling factor? For example, how...
Nikolay Boev 41 Feb 13 '14 at 04:45

Status of Wiki

1 0 5k
answers votes views
I'm just wondering what the status is of the wiki portion of this site. I receive error messages when trying to edit a ...
Ulrich Haunz 210 Feb 10 '14 at 01:38

using chenomx and missing peaks

1 0 3k
answers votes views
hello, I am trying to identify and quantify of metabolites present in semen with spectra obtained from 400 MHz NMR using...
Ulrich Haunz 210 Feb 10 '14 at 01:36

can topspin 3.2 window version open broker fid file?

3 0 4k
answers votes views
I installed the Topspin 3.2 pl 3 on windows 7, but when I am trying to open my NMR data it can only show process data fi...
John Hollerton 96 Jan 23 '14 at 09:36

Running a 13C{19F} spectrum on a Bruker Instrument?

1 0 6k
answers votes views
My apologies in advance for the very "beginner" nature of my question : ) We are interested in examining a carbon spect...
Charlie Fry 91 Jan 23 '14 at 07:32

2D ROESY-NMR with ~0 peak intensity

1 0 3k
answers votes views
I have been trying to elucidate the 3D structure of oxidized glutathione (M.Wt= 612.63 g/mol) with the help of roesy dis...
marcs 11 Jan 20 '14 at 22:46

how to read a file generated by SPARTA+

0 0 5k
answers votes views
i want to ask how to analyze a pred. file generated as a result of running SPARTA+ shift prediction software . ...
prashantiitb 1 Jan 20 '14 at 04:34

spin lock 1H spectrum

2 0 4k
answers votes views
Please, if somebody wants to help me?! I need to get 1H spectrum with spin lock pulse on Bruker AV 600 instrument. Which...
gugica 1 Jan 17 '14 at 05:06

residual dipolar coupling

1 0 2k
answers votes views
Hi, which aminoacid is best for calibrating acrylamide gel percentage for RDC? ...
dejian 11 Dec 29 '13 at 13:53

How to calibrate pulse length for urine samples?

2 0 5k
answers votes views
I recently read an article about executing a WET sequence for water suppression in urine samples. The 90°excitation puls...
brighman 1 Dec 26 '13 at 18:10

error while running humpcal

1 0 4k
answers votes views
Dear all, My new avance 400 is driving me crazy at the moment ! Shims are awful although I tried the topshim 3D on sucro...
univp5 1 Dec 20 '13 at 02:44

1-2 yrs funding to complete Ph.D. in Chemistry (Ionophores NMR Molecular Modelling)

0 0 6k
answers votes views
Looking for 1-2 yrs funding to complete my Ph.D. in Chemistry. Thesis: 'A Study of Conformational Analysis and Molecular...
craigofw 1 Dec 18 '13 at 08:15

how to do peak picking manually in VNMRJ

1 0 2k
answers votes views
I known how to pick peaks automatically. But it always omits some small peaks and picks some noise as peaks. Is there an...
dejian 11 Dec 12 '13 at 08:20

NMR Vs X ray crystal data not matching each other

2 0 3k
answers votes views
I had a very weird situation where my compound shows double bond between carbons in NMR but shows it as single bond in x...
John Hollerton 96 Dec 09 '13 at 09:57

Best way to make a 2D figure

2 0 5k
answers votes views
Hi all, I need to make a figure containing a 2D NMR spectrum for a paper. Has anyone figured out a good way to do this...
John Hollerton 96 Dec 09 '13 at 09:44

Relative quantification (residual solvents)

2 0 33248
answers votes views
Hi All, I've been trying to get a clear answer to this question for a while now, but can't seem to get a definitive ...
John Hollerton 96 Dec 09 '13 at 09:37

how to get a 1H-15N HMBC spectroscopy

4 0 8k
answers votes views
hi All, I run a 1H-15N HMBC experiment on a Bruker AVANCE III 600 MHz spectrometer. But I fail to get the long- range ...
Kirk Marat 711 Dec 09 '13 at 07:48

Streaks in RDC Gel Spectrum

2 0 8k
answers votes views
Hello all, I have used stretched polyacrylamide gels successfully many times to collect RDC data for proteins. The las...
ChemMJW 99 Dec 06 '13 at 19:10

"Satellite" peaks in polypeptide HSQC

1 0 3k
answers votes views
In the decoupled HSQC of a polypetide I see two small peaks right and left to the delta arginine signal. These peaks hav...
Pete Gierth 401 Nov 29 '13 at 00:45

PHIP experiments

0 0 1k
answers votes views
Dear all! I am struggling with some density matrices in PHIP experiments. I understand why the density matrix before hyd...
Elina Lisitsin 1 Nov 28 '13 at 02:41

How to exactly set integral start and end in VNMRJ

0 0 1k
answers votes views
One can roughly set the integral start and end by left clicking in the spectra. Is there a command which let you type in...
de xiao 11 Nov 27 '13 at 07:58

31P signal at negative 74 ppm

2 0 7k
answers votes views
Hello All Does anyone have any ideas on what might give a phosphorus signal at negative 74 ppm (cf phosphoric acid)? T...
Craig 41 Nov 19 '13 at 05:11

why prefer using Nitrogen NMR than carbon and hydrogen NMR

1 0 2k
answers votes views
Hi, I am new in NMR. Can someone please answer me why prefer N-NMR but not C and H -NMR? Thanks ...
de xiao 11 Nov 18 '13 at 07:50

NMR of compounds containing iron

1 0 3k
answers votes views
Are there any problems with doing proton NMR of a compound containing iron? ...
Debbie 1 Nov 15 '13 at 10:43

How to convert inversion recovery data acquired using Spinsight

0 0 3k
answers votes views
Hi, I do not know how to convert a data set acquired using Spinsight into a format that can be read by Topspin. The d...
mike 1 Nov 14 '13 at 14:00
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