i like this post (click again to cancel)
0
i dont like this post (click again to cancel) remove favorite mark from this question (click again to restore mark)

I have some older 2D HSQC 1H-15N data that was run by a NMR tech. Is there a way I can tell what kind of solvent suppression was used by looking through the acquisition files? Like I know I can open them up as text documents and see a lot of the experimental parameters, but I don't know if solvent suppression is listed in one of those parameters.

Thanks! -Brian W.

asked Nov 22 '13 at 20:05

BrianW's gravatar image

BrianW
1


2 Answers:
i like this answer (click again to cancel)
0
i dont like this answer (click again to cancel)

The easiest thing to do is to look at the pulse sequence directly. You didn't mention whether you're using Bruker or Varian (Agilent) spectrometers, but both TopSpin and VNMR/VNMRJ can display the pulse sequence graphically, from which it's fairly easy to identify the water suppression method. Alternatively, you can look at the text file of the pulse program itself. Many standard pulse programs have comments at the very beginning that give the general outline of how the sequence works, and the method of water suppression is often given as part of this header data. Furthermore, many pulse sequences are labeled by name (comments in the actual code) at the start of common blocks such as INEPT, etc. Maybe you can find info on the water suppression method there.

Unless you're running an exotic flavor of HSQC experiment, chances are you're dealing with a common water suppression method such as WATERGATE or a water flip-back pulse.

link

answered Nov 22 '13 at 21:19

ChemMJW's gravatar image

ChemMJW
99

i like this answer (click again to cancel)
0
i dont like this answer (click again to cancel)

If it's a Bruker sequence, then there should be a file called pulseprogram in the directory containing the data. You can use any kind of text editing software (Wordpad, nedit, gedit, etc) to view the file. It should specifically say what kind of solvent suppression was used.

link

answered Feb 26 '14 at 07:11

uabnmr1's gravatar image

uabnmr1
1

Your answer
Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)
toggle preview

powered by CNPROG