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Can anybody provide parameter file for running 1 D STD saturation transfer difference experiment with DPFGSE sculpted solvent suppresion on varian 600 MHz instrument.

asked Sep 23 '09 at 06:49

sns03003's gravatar image

sns03003
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updated Sep 28 '09 at 12:15

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
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looks like there is macro satxfer_dpfgse if you call it, most parameters will be set correctly.

I've set up a simple manual page, which lists the adjustible parameters for this sequence.

Also, if you are curious you can read the text of the pulse sequence: type

more /vnmr/psglib/dpfgse_satxfer.c

in the UNIX command line (assuming that your system files are in /vnmr directory). There are three literature references that describe details of the sequence.

Probably the less usual part of the sequence is that it uses fine power control for the solvent-selective 180 pulse (associated with parameters wrefpw, wrefshape, wrefpwr, wrefpwrf).

To start with - set wrefpwr to the level you would normally use for a 180 of duration wrefpw and shape wrefshape, and wrefpwr=2048 (default value). Then adjust wrefpwr if you do not have freedom in choosing wrefpw (most often it is not necessary), otherwise just fine-tune wrefpw so that pulse becomes 180. Default recommended wrefshape is square (you probably have a file called shapelib/square.RF on your system).

link

answered Sep 28 '09 at 12:07

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5571

updated Feb 05 '10 at 08:21

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