i like this post (click again to cancel)
1
i dont like this post (click again to cancel) remove favorite mark from this question (click again to restore mark)

Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the moment from looking at the Biopack library, I have found 4 pulse sequences which will do this and each provide different information (gnoesyNhsqc_CN, gnoesyNhsqc_NN, gnoesyChsqc_CC and gnoesyChsqc_NC). Would people recommend using a combination of all four of these experiments to determine the intermolecular NOEs for use in structure calculations?

asked Aug 09 '10 at 17:47

acalab's gravatar image

acalab
21

updated Aug 09 '10 at 17:54

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771


One Answer:
i like this answer (click again to cancel)
0
i dont like this answer (click again to cancel)

Hmm, this is really odd, but in our deployment of BioPack the sequences you are mentioning have been replaced with the following statement (or similar):

/*  gnoesyChsqc_NC   for Heterodimer Studies
    has been temporarily removed
    for pulse sequence revision
*/

I've found the following isotope-filtered sequences in the library:

CNfiltocsy.c          gnoesyChsqc_CNfilt.c
gChmqcnoesy_CNfilt.c  gnoesyNhsqc_CNfilt.c

Also, according to the Varian/Agilent user library (you'll have to find the biopack page there and register first) the noesy-hsqc sequences were updated in Dec 2009.

link

answered Aug 10 '10 at 14:45

Evgeny%20Fadeev's gravatar image

Evgeny Fadeev
5771

updated Aug 10 '10 at 16:52

Your answer
Please start posting your answer anonymously - your answer will be saved within the current session and published after you log in or create a new account. Please try to give a good answer, for discussions, please use comments and please do remember to vote (login to vote)
toggle preview

powered by CNPROG