Examples of protein degradation eg HSQC?

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Anyone know any websites or papers that show protein degradation in an HSQC for example? I wanted to know what to look for in old samples when I have them. Either that, or could someone put one up on the wiki for me to look at?

degradation protein

asked Jul 13 '10 at 02:55

madge
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updated Aug 08 '10 at 16:13

Evgeny Fadeev
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When I get degredation of my protein samples I look out for very sharp peaks towards the botom centre of my spectra say around 8 by 126 ppm, I tend to see them running through my HSQC at around 8 ppm but the one at 126 ppm in the 15N seems to always be there when I get break down whatever the protein, ther usualy much sharper than say even your random coil regions like speckles runnig through your spectra.. as you might expect.

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answered Jul 13 '10 at 04:46

TomgA20
61

Thank you. Very helpful. - madge (Jul 18 '10 at 23:56)

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Asked: Jul 13 '10 at 02:55

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Last updated: Aug 08 '10 at 16:13

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