Tagged questions

Observing protein-ligand interaction using weakly soluble compounds

1 0 7k
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I am trying to setup an experiment using HSQC with titration of a compound to determine the Kd of the protein-ligand int...
Justin Frank 1 Jun 29 '16 at 19:39

How to calcualte eoors in ppm from Hz of Hsqc data?

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Hi all, I want to calculate error in ppm from my Hsqc data. My spectrometer yields X1axis, Y1Axis, DX1, DY1, X1ppm, and ...
Arkadiusz Leniak 238 Mar 17 '15 at 02:10

TOCSY of peptide in micelle

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Hi, I am a PhD student starting in NMR and I have two questions. I have a problem with a TOCSY and natural abundance ...
paul atray 1 Sep 17 '14 at 10:16

Which Bruker HSQC program do you use?

5 0 4k
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Hello, could you tell me which HSQC and E-HSQC program are recommendable ? These are Bruker HSQC pulse programs: hsqcp...
RuudA 11 Sep 10 '14 at 11:46

Streaks in RDC Gel Spectrum

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Hello all, I have used stretched polyacrylamide gels successfully many times to collect RDC data for proteins. The las...
ChemMJW 99 Dec 06 '13 at 19:10

"Satellite" peaks in polypeptide HSQC

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In the decoupled HSQC of a polypetide I see two small peaks right and left to the delta arginine signal. These peaks hav...
Pete Gierth 401 Nov 29 '13 at 00:45

hsqc referencing

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Hi! I need to know how to reference nitrogen 15 from proton in a foldel hsqc experiment...I don't know how to calculate ...
sekhar Talluri 621 Nov 01 '13 at 17:46

The FID in the F1 dimension is not fully sampled – it is cut off.

0 0 4k
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HELLO all nmr wikier, I am facing problem in hsqc experiment on bruker machine using pulse prog hsqcetgpsi Normally the ...
feroon 21 Jun 27 '13 at 10:09

peak missing in nh hsqc protein nmr

4 0 16342
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Dear NMR wikiers I am assigning 95 aminoacid residue protein . I can see 76 cross peaks in N H HSQC on 700 vnmr cryob ...
sri 71 Aug 25 '11 at 00:05
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