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Hi! I need to know how to reference nitrogen 15 from proton in a foldel hsqc experiment...I don't know how to calculate the new 'lowest frequency' from the parameters I have in the processed spectrum... could someone help me? thanks!! |
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Hi Silvia, You should be able to get the correct chemical shift by simply adding or subtracting one full spectral width. |
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The IUPAC-IUB recommended chemical shift referencing ratios for indirect chemical shift referencing are available at : http://bmrb.wisc.edu/ref_info/cshift.shtml For anydrous liquid ammonia it is 15N-1H (DSS) 0.101329118 BTW, the method of conversion of folded frequencies into corrected frequencies depends upon the method used for quadrature detection in the indirect dimension: TPPI, States or States-TPPI. If quadrature detection is used according to the method of States et al., then the carrier frequency is at the center and the ends of the processed spectrum are at carrier frequency +/- SW/2 in the indirect dimension. If TPPI is used for the indirect dimension then, the carrier frequency is at one end of the spectrum (if the processed spectrum is oriented according to common conventions, it will be at the bottom) in the indirect dimension. |
