Unanswered questions

Observing protein-ligand interaction using weakly soluble compounds

1 0 8k
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I am trying to setup an experiment using HSQC with titration of a compound to determine the Kd of the protein-ligand int...
Justin Frank 1 Jun 29 '16 at 19:39

Can anyone help me with Saturation Transfer NMR

2 1 5k
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Hi I am working on a Varian 500MHz NMR. I am attempting to use the pulse sequence satxfer1D to measure the rate constant...
Justin Frank 1 Jun 29 '16 at 19:30

saturation transfer method in NMR

2 1 9k
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What is meant by saturation transfer method in NMR? How it is useful in giving the structure of blue copper proteins lik...
Justin Frank 1 Jun 29 '16 at 19:26

selecting for side chains

2 1 3k
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Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2? ...
Justin Frank 1 Jun 29 '16 at 19:22

Dimer and tetramer equilibrium identification of protein with NMR experiments

2 0 8k
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Dear nmr wikiers could you please let me know is their any nmr experment to identification of Dimer and tetramer equil...
Justin Frank 1 Jun 29 '16 at 19:09

N15 NNH separated NOESY

2 0 4k
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Dear friends, I came across one reference which describe N15 NNH separated NOESY experiment but I am not able to get ...
Justin Frank 1 Jun 27 '16 at 22:53

Protein-Protein Interactions

1 0 2k
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Hi i'm a newbie in NMR and I have 2 proteins, A protein is about 12KDa, B protein is about 45 KDa,i know that A protein ...
Justin Frank 1 Jun 27 '16 at 22:46

peptide protein interaction

6 1 9k
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I have determined the structure of a 110 residues protein(11kDa) which is known to interact with a 15mer peptide. Now I ...
Justin Frank 1 Jun 27 '16 at 22:44

A beginner's guide to protein backbone assignment?

3 0 7k
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Hi All, I'm new to protein backbone assignment by NMR, and my protein is about ~37kDa (about 400 residues). We've acqui...
Justin Frank 1 Jun 27 '16 at 21:03

NMR of proteins

3 0 6k
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I need informations about nuclear magnetic resonance of proteins with figures. ...
Justin Frank 1 Jun 27 '16 at 20:47
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