A beginner's guide to protein backbone assignment?

3 0 6k
answers votes views
Hi All, I'm new to protein backbone assignment by NMR, and my protein is about ~37kDa (about 400 residues). We've acqui...
Justin Frank 1 Jun 27 '16 at 21:03

NMR of proteins

3 0 5k
answers votes views
I need informations about nuclear magnetic resonance of proteins with figures. ...
Justin Frank 1 Jun 27 '16 at 20:47

What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?

2 1 11702
answers votes views
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a str...
Justin Frank 1 Jun 27 '16 at 19:27

Protein-peptide binding NMR Experments

2 0 7k
answers votes views
Hello friends , my target Protein is existing as multimer (i.e Monomer,dimer,tetrameter ) before binding to the peptid...
Justin Frank 1 Jun 27 '16 at 03:02

Difference between H 50 W and X 100 W Amplifiers

2 0 3k
answers votes views
We have been trying to get a 2D H-F experiment to work using the X 100 W amplifier on our Bruker NMR, but can't get it t...
Kirk Marat 711 Jun 14 '16 at 11:55

When to use 'BLKGRAD' and 'UNBLKGRAD' in a bruker pulse sequence ?

1 0 2k
answers votes views
I'm trying to modify some pulse sequence on bruker 400, and is confused with the use of 'blkgrad' and 'unblkgrad'; and ,...
Kirk Marat 711 Jun 14 '16 at 11:44

method validation in nmr

1 0 2k
answers votes views
please tell me whether there are method validation in nmr ...
Ulrich Haunz 210 May 04 '16 at 01:31

Shimming for liquid stateNMR

1 0 1k
answers votes views
Dear All, What should be the intesnities of spinning sides bands in liquid state NMR?. THank YOu ...
Clemens Anklin 416 Nov 27 '15 at 11:26

reversed NOESY/EXSY peaks

1 0 2k
answers votes views
We have a case of a molecule that has two slowly exchanging conformations. In the NOESY experiment we see cross-relaxing...
Pete Gierth 401 Nov 23 '15 at 11:26

1H 1D NMR data Normalization in Topspin.

1 0 3k
answers votes views
I have performed a series of 1H 1D experiments with different concentration of peptide and accordingly different numbers...
Pete Gierth 401 Nov 23 '15 at 11:14
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