incorporating fluorine into proteins

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I was considering the possibility of trying some fluorine NMR and was wanting to know if anyone knew any simple ways of incorporating Fluorine probes into my protein relatively cheaply or using E.coli. I would be particularly interested in using fluro-tryptophan for dual use as NMR and florescence probes. Also does anyone have a feel for the size limitations of 19F probes literature always quotes around 100KDa but is it possible to go considerably higher, say 500?

any advice would be great

Tom

19F proteins

asked Feb 02 '12 at 12:14

Thomas Garner
81

2 Answers:

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0	You could look at Carl Frieden's work. I remember his work on fluoro-tryptophan and fluorine NMR. Like this - http://pubs.acs.org/doi/abs/10.1021/bi00184a019 ? link answered Feb 05 '12 at 06:00 service email start 11 updated Feb 05 '12 at 06:02

0	recently a paper published in Chem Comm by Crowley group: "Simple and inexpensive incorporation of 19F-Tryptophan for protein NMR spectroscopy" I used this protocol and got 19F labeled protein (not big size protein, though). link answered Nov 13 '15 at 13:33 sdc 1

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Asked: Feb 02 '12 at 12:14

Seen: 4,714 times

Last updated: Nov 13 '15 at 13:33

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