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I was considering the possibility of trying some fluorine NMR and was wanting to know if anyone knew any simple ways of incorporating Fluorine probes into my protein relatively cheaply or using E.coli. I would be particularly interested in using fluro-tryptophan for dual use as NMR and florescence probes. Also does anyone have a feel for the size limitations of 19F probes literature always quotes around 100KDa but is it possible to go considerably higher, say 500?

any advice would be great


asked Feb 02 '12 at 12:14

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Thomas Garner

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You could look at Carl Frieden's work. I remember his work on fluoro-tryptophan and fluorine NMR. Like this - http://pubs.acs.org/doi/abs/10.1021/bi00184a019 ?


answered Feb 05 '12 at 06:00

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updated Feb 05 '12 at 06:02

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recently a paper published in Chem Comm by Crowley group: "Simple and inexpensive incorporation of 19F-Tryptophan for protein NMR spectroscopy" I used this protocol and got 19F labeled protein (not big size protein, though).


answered Nov 13 '15 at 13:33

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