poor signal to noise on 1H-15N heteronculear NOE

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I have recently attempted to run a 1H-15N heteronuclear NOE experiment in vnmrj (700 rt probe) using the standard sequence that comes with biopack, the signal to nose im getting compared to my standard HSQC is significantly reduced, is this normal? I do not recall this being the case when I ran this previously on a bruker 600 with RT probe? is this normal or does this suggest something is miss set? Also in the no NOE experiment I get very broad noise in the centre of my spectrum/water line.

regards

Tom

signal-to-noise noe

asked Oct 31 '11 at 07:38

Thomas Garner
81

2 Answers:

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1	hsqc uses polarization transfer to magnify signal, while NOE uses NOE effect. Their enlarged signals come from different reasons. I think it is normal to find S/N reduced. May be ten times. regards, Jing Zhang link answered Nov 06 '11 at 19:32 jinghere 11

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"May be ten times." Sorry this is wrong. The reason is 15N has a negative NOE effect, leading weaker signal.

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answered Nov 09 '11 at 02:43

jinghere
11

I understand that its just that both with and without NOE give me very poor signal but i guess your previous answer explains that also, thank you - Thomas Garner (Nov 09 '11 at 08:48)

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Asked: Oct 31 '11 at 07:38

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Last updated: Nov 09 '11 at 08:48

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