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Hello nmr wikier I want to run pulprog h2bcetgpl3 on bruker instrument using adiabiatic shape pulse crp60comp.4 I determine power level sp7 in stdisp for p24 2ms on rga it is warning that d28 is negative how i adjurst this parameter. thanks for answering in advance with best regard feroon |
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It is most likely that the d21 parameter has not been set (this is the proton-proton coupling evolution delay). Typically this would be 22 ms. D28 is calculated from this parameter (d21) so if d21 is zero you will see the error you describe. |
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Thanks for replying Tim claridge, I have set d21 as it is in pulseprog[16-22 msec] 22ms and tried on 20ms then 16ms but this is warning that d28 is negative and rga aborated. Hello feroon, please use comment feature next time to follow up, or edit your original question. This forum is made for questions and answers so that it is easier to follow, otherwise content becomes too cluttered. Cheers. - Evgeny Fadeev (Sep 24 '11 at 11:43) |
Could you successfully run the H2BC expt. I face the same d28 being negative problem. Thanks. - Damodaran Krishnan Achary (Oct 15 '11 at 18:07)
No I am still awaiting some hints from anywhere. - feroon (Oct 17 '11 at 07:44)
As a new NMR user I've encountered the same issue. The solution is to set proper amount of increments in t1 (not higher than 128). I use t2,t1 = 2k,128; SW (10 ppm, 195 ppm) and d21=20 msec. - zablociak (May 30 '14 at 13:21)