Setting up J-HMBC experiment (hmbcetgpjcl2nd)

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Hi, i'm trying to set a J-HMBC experiment to measure coupling constant J2-J3 CH (hmbcetgpjcl2nd) to determine stereochemistry of organic compounds in a Bruker but I can not set the parameters correctly. Someone could put at least the basic parameters to perform this experiment?

Thanks in advanced.

j-hmbc scalar-coupling stereochemistry

asked Jul 09 '10 at 12:47

Cleber Barreto
73

updated Jul 09 '10 at 17:37

Hi Cleber, could you email me the pulse-sequence? I'll document it on the wiki evgeny.fadeev@gmail.com. Will just post documentation, not the code so that authors will not be upset. - Evgeny Fadeev (Jul 09 '10 at 16:36)

I just send the pulses sequence to you. Thanks. Any help is welcome. - Cleber Barreto (Jul 09 '10 at 17:34)

got your file, will post documentation by tuesday. - Evgeny Fadeev (Jul 10 '10 at 09:41)

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Hi Cleber, here is the manual to the sequence that you want to run that I've set up after reading the sequence and the paper. The sequence corresponds to figure 1B of the article cited within. I did not run the sequence myself though.

link

answered Jul 12 '10 at 13:43

Evgeny Fadeev
577●1

updated Jul 12 '10 at 13:50

thank you very much for the manual. Will help a lot. However, I was not sure at what value of cnst13 should I use? From what I saw in the paper, values cnst6 and cnst7 can be 125 and 165 respectively, but does not mention anything of value cnst13. - Cleber Barreto (Jul 12 '10 at 15:34)

Hi. Reading more carefully I realized that the value should be 1 Hz in cnst13 for d6 (DELTA) is longer than 500 ms. Thanks for the help. - Cleber Barreto (Jul 13 '10 at 02:47)

Thanks for the clarification, I've missed that part. - Evgeny Fadeev (Jul 13 '10 at 13:57)

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scalar-coupling ×5
j-hmbc ×1
stereochemistry ×1

Asked: Jul 09 '10 at 12:47

Seen: 13,903 times

Last updated: Jul 12 '10 at 13:50

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