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Best pulse sequence for HSQC in Guanidine-HCl

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Hello, I would like to acquire an HSQC spectrum of an 15N-labeled 12 kDa protein unfolding (over the course of hours) i...
Posted: vadim 23 / Updated: Pete Gierth 326 Oct 30 '10 at 06:29

Which are better methods of water suppression at higher salt concentrations?

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Hello, could anyone recommend better technique for water suppression for the samples having higher salt concentration? ...
Updated: Evgeny Fadeev 5571 Jul 26 '10 at 09:26

Why is water suppression is harder to achieve in salty samples?

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Tried an excitation sculpted watergate on a sample in H2O with 150 mM salt and I found that it is very hard to suppress ...
Posted: Evgeny Fadeev 5571 / Updated: sekhar Talluri 601 Jul 11 '10 at 02:44
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