RDC gel preparation

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I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the following 1)drying the gel and re-swelling with protein solution 2)allowing equilibration of protein into an already swelled gel

It seems as though you can get a much higher protein conc. if you dry the gel so what is the advantage to letting it equilibrate?

Thanks

asked Jan 24 at 08:42

andrewL
1

Um.... Is this an NMR question? - Kirk Marat (Jan 26 at 11:06)

It is an NMR sample preparation question for residual dipolar coupling experiments (IPAP). - andrewL (Jan 27 at 07:03)

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0	I have heard that people use the soaking into a swelled gel method to avoid introducing inhomogeneity in the gel due to drying. I use the drying method, but I have seen that it can cause the gel to get a bubble in it. link answered Jan 30 at 07:01 mikaelastewart 11

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protein ×22
nmr ×13
rdc ×7
gel ×1

Asked: Jan 24 at 08:42

Seen: 195 times

Last updated: Jan 30 at 07:01

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