Unanswered questions

Why do unpaired electrons make NMR measurements difficult?

2 1 9k
answers votes views
Can someone please answer this for me? ...
Posted: TShpiruk 11 / Updated: Partha Mukherjee 1 Sep 27 '11 at 06:43

h2bc experiment

2 1 6k
answers votes views
Hello nmr wikier I want to run pulprog h2bcetgpl3 on bruker instrument using adiabiatic shape pulse crp60comp.4 I determ...
Posted: feroon 21 / Updated: zablociak 21 May 30 '14 at 13:21

Beginners question: checking probe and multinulear ability in Bruker machines

2 1 14084
answers votes views
Hi All, This may be a daft question, but how do I go about identifying the probe in our NMR machine, and hence whether ...
Posted: blankchem 11 / Updated: Kirk Marat 711 Jul 04 '14 at 08:51

Can anyone help me with Saturation Transfer NMR

2 1 5k
answers votes views
Hi I am working on a Varian 500MHz NMR. I am attempting to use the pulse sequence satxfer1D to measure the rate constant...
Posted: njmagarian 11 / Updated: Justin Frank 1 Jun 29 '16 at 19:30

T1 Rho relaxation

2 0 9k
answers votes views
I am looking for some guidance on researching T1rho relaxation. Does anybody have updated information or references to e...
Posted: Robert Bussell 1 / Updated: Alexey Potapov 21 Oct 15 '11 at 04:56

Side chain assignment of C-terminal residue

2 0 4k
answers votes views
Dear Friends, I am not able to figure out how to determine the side chain assignment of Last C-terminal SERINE residue...
Posted: Arun Gupta 11 / Updated: Andrew Fowler 96 Oct 11 '11 at 09:24

Using CYANA and ARIA to caluclate structure of protein

2 0 4k
answers votes views
Could you please describe about advantages of CYANA2.1 and ARIA1.2 software to calculate the structure of protein ? w...
Updated: sri 71 Oct 28 '11 at 03:01

Protein-peptide binding NMR Experments

2 0 7k
answers votes views
Hello friends , my target Protein is existing as multimer (i.e Monomer,dimer,tetrameter ) before binding to the peptid...
Posted: sri 71 / Updated: Justin Frank 1 Jun 27 '16 at 03:02

poor signal to noise on 1H-15N heteronculear NOE

2 0 4k
answers votes views
I have recently attempted to run a 1H-15N heteronuclear NOE experiment in vnmrj (700 rt probe) using the standard sequen...
Updated: Thomas Garner 81 Nov 09 '11 at 08:48

EBURP-2 wavelib shape pulse file for varian

2 0 5k
answers votes views
Can somebody please send the EBURP-2 wavelib file for VNMR? Thanks! AL ...
Posted: Avigdor Leftin 1 / Updated: jinghere 11 Nov 13 '11 at 23:18

what went wrong?

2 0 3k
answers votes views
a query again? I recorded an DQF on deuterated solid sample. The basic pulse programme taken from bruker, optimised the...
Updated: anup chowdhury 1 Nov 21 '11 at 07:34

demonstrative kinetics experiment

2 0 4k
answers votes views
I'm looking for a good kinetics experiment to run in the lab section of our graduate lab course. It should be a reaction...
Posted: jkurutz 131 / Updated: sekhar Talluri 621 Dec 03 '11 at 13:32

Odd distortion in HOD peak in 1D DOSY (stebpgp1s)

2 1 4k
answers votes views
I have a small distortion in the HOD peak in my 1D DOSY spectra. However, it only occurs for the HOD signal and no other...
Updated: cyg 36 Jan 03 '12 at 21:45

bulk water relaxation dependence on temperature

2 1 6k
answers votes views
Is liquid water's relaxation rate strongly dependent on temperature, and does anyone have a link to a good online articl...
Posted: plshelp 11 / Updated: sekhar Talluri 621 Jan 15 '12 at 09:58

Data processing and Sparky

2 0 5k
answers votes views
Dear NMRWikiers, I'm fresh in nmr field and would like to ask couple questions: 1. Our protein has about 132 residues.Th...
Posted: John Leehono 1 / Updated: Justin Frank 1 Jun 29 '16 at 19:44

incorporating fluorine into proteins

2 0 4k
answers votes views
Hi I was considering the possibility of trying some fluorine NMR and was wanting to know if anyone knew any simple ways...
Posted: Thomas Garner 81 / Updated: sdc 1 Nov 13 '15 at 13:33

N15 NNH separated NOESY

2 0 4k
answers votes views
Dear friends, I came across one reference which describe N15 NNH separated NOESY experiment but I am not able to get ...
Posted: Arun Gupta 11 / Updated: Justin Frank 1 Jun 27 '16 at 22:53

PC rebooted suddenly and Topspin got problem. Has this happened to you?

2 0 4k
answers votes views
Hello all, I was measuring on a Topspin 300MHz and multizg was running (T1ir and cpmg). I unplugged my usb flash memory ...
Updated: Amin os 43 Mar 14 '12 at 02:30

set up aromatics experiment in VNMRJ2.2

2 1 3k
answers votes views
Dear Sir, I am new to set up of aromatic assignment experiment. Can you please specify what parameters in VNMRJ 2.2 a...
Updated: Arun Gupta 11 Apr 05 '12 at 22:42

Why Wobb for 19F is so much dependant on the kind of sample while 1H not?!!

2 0 5k
answers votes views
Normally for 1H, it doesn't matter so much to tune the probe with or without a sample inside. And during the measurement...
Updated: Amin os 43 Jun 06 '12 at 07:26

Why do we turn off sample spinning when we do NOESY experiment?

2 1 6k
answers votes views
Why do we trun off sample spinning when we do NOESY experiment? ...
Posted: Kiu Chor Sham 11 / Updated: Evgeny Fadeev 5771 Jun 03 '12 at 17:23

Unexpected Echo intensity increase in DOSY

2 2 4k
answers votes views
Hi, I ran a typical dosy expt and got an unexpected increase on echo intensity in the begining of the array. The first ...
Posted: fid 139 / Updated: mikaelastewart 11 Jun 03 '13 at 09:14

selecting for side chains

2 1 3k
answers votes views
Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2? ...
Posted: service email start 11 / Updated: Justin Frank 1 Jun 29 '16 at 19:22

help on creating a program similar to multizg?

2 1 6k
answers votes views
I am currently trying to write a program that is a lot like the multizg program in the Bruker software, Topspin but with...
Posted: Artjom Nepryahin 11 / Updated: John Hollerton 96 Dec 10 '12 at 10:05

eretic standard sample

2 1 6k
answers votes views
I have recently set up the eretic sample concentration measurement experiment experiment on our AvanceIII instrument. ...
Posted: Matthew Revington 41 / Updated: John Hollerton 96 Dec 10 '12 at 08:53

ACD/NMR vs. MestReNova calculation difference

2 0 5k
answers votes views
Hi there, how is possible that peak area calculated by ACD/NMR is lower than the same one calculated by MestReNova soft...
Posted: Frantisek Surman 1 / Updated: Kirk Marat 711 Jun 20 '12 at 13:45

STDtrNOESY

2 0 3k
answers votes views
Hi Eeveybody, Is there anyone who can help me in processing the STD-Tr-NOESY 2D spectrum, i have done the experiment bu...
Posted: john dawn 1 / Updated: renee 56 Jul 31 '12 at 17:45

Lock at high temperatures

2 1 7k
answers votes views
I am having trouble locking a sample of a polymer dissolved in OCDB at 135 degree's. Can this high temperature interfer...
Posted: Javaslinger 53 / Updated: Gareth Morris 71 Jul 08 '12 at 03:02

How important is calibrating a 90degree pulse for 2D?

2 1 4k
answers votes views
So it is pretty much always recommended to calibrate the 90 for 1H for any 2D experiment... Cosy, Noesy, HSQC, etc... ...
Posted: Javaslinger 53 / Updated: Nikolay Boev 41 Jun 29 '12 at 09:04

Lock Error Bruker Ascend 600

2 0 11371
answers votes views
Hi there, We had a short power failure yesterday and since my boss is in Ireland and our other so called NMR guru does...
Posted: Patrick Seibert 1 / Updated: aaaeee 1 Apr 12 '18 at 12:50
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