interleaved acquisitionon Bruker NMR

2 0 5k
answers votes views
Our group just transfered from Varian to Bruker NMR, and we have a problem. Since my sample is unstable, when thereis a ...
Debashish Sahu 1 Feb 16 '17 at 14:27

eightfold suppression of water and ethanol

0 0 4k
answers votes views
Hi, I'm trying to perform an NOESYGPPS 1D 1H NMR experiment to suppress water and the ethanol signals in a wine sample, ...
Calin Floare 1 Feb 06 '17 at 05:15

Why is the T1 relaxation time of protons shorter than for other nuclei?

0 0 5k
answers votes views
So I think this could be asked in my oral exam. Why are the T1 relaxation times in NMR spectroscopy shorter than those o...
NMR Dummy 1 Jan 25 '17 at 09:56

Protein Backbone assignment

1 0 4k
answers votes views
There are many Software Tools available for that purpose. What are the problems there (if there are some)? Do you think...
FAS 1 Dec 08 '16 at 07:53

Effect of components of B1 field

0 0 2k
answers votes views
Considering the B0 field is along Z direction if and B1 is applied in the XY plane. What will be the effect on SNR of an...
Divine Demon 1 Dec 06 '16 at 04:05

Backbone assignment with NMRPipe

1 0 3k
answers votes views
Hi I'm new to nmrPipe and I have a question about where to start assigning the backbone resonances for a recombinant pro...
Woonghee 26 Nov 28 '16 at 16:37

How to implement receiver phase toggle in XWINNMR?

1 0 3k
answers votes views
How does one change the receiver phase DURING stroboscopic acquisition in XWINNMR? The "ipp" commands do not seem to wor...
forza 1 Nov 10 '16 at 10:22

NOE and strength of dipolar coupling

1 0 3k
answers votes views
Is NOE observed even if molecular motion averages out the dipolar coupling between two spins. ...
Kirk Marat 711 Oct 26 '16 at 15:01

qNMR calculation

1 0 13082
answers votes views
I am currently working on the qNMR concentration of compounds X in commercial samples: The following is my scenario I...
Ulrich Haunz 210 Oct 13 '16 at 06:38

2rr to ASCII

2 0 3k
answers votes views
Hi All, I was wondering if there is a simple way for converting 2rr to ASCII format (with Origin?). I don't know if my ...
Kirk Marat 711 Sep 28 '16 at 08:07

NMRLab or matNMR for 1D nmr spectra processing?

1 0 2k
answers votes views
Hi, I used to use spinworks to process simple 1D NMR (because it was easy and I didn't felt like paying for topspin). B...
Kirk Marat 711 Sep 27 '16 at 07:21

Vnmr spectrum file

1 0 3k
answers votes views
Hi, I am working on software for NMR assignment and one of the options to implement is import Varian spectra into our ap...
Ulrich Haunz 210 Sep 19 '16 at 06:59

unresolved signals

0 0 2k
answers votes views
Hi all We know that H NMR of paramagnetic sample or complex, is very broad with a bad signal to noise ratio. Do you...
Ulrich Haunz 210 Sep 19 '16 at 06:51

FILTERATION OF SAMPLE

1 0 3k
answers votes views
Hello all, can i filter sample by passing the sample through a cotton? I think may be some of compound in cotton dis...
Ulrich Haunz 210 Sep 12 '16 at 05:56

HNCO projection of the 1H-15N plane

1 0 2k
answers votes views
Dear all, If I make the projection of the 1H -15N plane from the HNCO and compare it to the HSQC of the same sample, the...
Kirk Marat 711 Sep 06 '16 at 11:30

What do -di and -verb do?

1 0 2k
answers votes views
Hi all, New to NMRPipe. I'm trying to understand what the arguments -di and -verb to, e.g. in |nmrPipe -fn PS -p0 66.0...
Kirk Marat 711 Sep 06 '16 at 11:23

2D-NOESY for macromolecules

0 0 2k
answers votes views
Hi, NOESY is very interesting method bet I have a problem. We are working with polymethacryles (MW about 20 000-30 000 ...
AnnaLT 1 Aug 24 '16 at 22:32

unusual tyrosine cross peaks

3 1 5k
answers votes views
Hi I am observing some unexpected cross peaks in some homonuclear protein data and I was wanting to know if anyone had a...
james han 1 Aug 01 '16 at 21:28

Relaxation rate of bulk (pure) water at high frequencies

1 0 3k
answers votes views
Hi all, Does anyone know if NMR relaxation rates of bulk (pure) water has been measured experimentally at several hundr...
Jerry Hirschinger 199 Jul 21 '16 at 05:08

Aminoacid residue identification or assignment of protein from 3D NMR Experments

3 1 6k
answers votes views
Dear NMR Wiki'ers I am identifying aminoacid residues or assignment of protein from following 3D NMR experments HNCA...
Justin Frank 1 Jun 29 '16 at 19:53

c13 edit noesy phasing problem

3 1 5k
answers votes views
Dear NMR Wiki'ers I Have acquired c13-edit noesy experiment on 700vnmr for my target protein (20kd) with mixing time of...
Justin Frank 1 Jun 29 '16 at 19:48

Data processing and Sparky

2 0 5k
answers votes views
Dear NMRWikiers, I'm fresh in nmr field and would like to ask couple questions: 1. Our protein has about 132 residues.Th...
Justin Frank 1 Jun 29 '16 at 19:44

Observing protein-ligand interaction using weakly soluble compounds

1 0 8k
answers votes views
I am trying to setup an experiment using HSQC with titration of a compound to determine the Kd of the protein-ligand int...
Justin Frank 1 Jun 29 '16 at 19:39

Can anyone help me with Saturation Transfer NMR

2 1 5k
answers votes views
Hi I am working on a Varian 500MHz NMR. I am attempting to use the pulse sequence satxfer1D to measure the rate constant...
Justin Frank 1 Jun 29 '16 at 19:30

saturation transfer method in NMR

2 1 9k
answers votes views
What is meant by saturation transfer method in NMR? How it is useful in giving the structure of blue copper proteins lik...
Justin Frank 1 Jun 29 '16 at 19:26

selecting for side chains

2 1 3k
answers votes views
Does anyone know of literature pointing to NMR experiments that can select for say -CH3 groups attached to -CH2? ...
Justin Frank 1 Jun 29 '16 at 19:22

Dimer and tetramer equilibrium identification of protein with NMR experiments

2 0 8k
answers votes views
Dear nmr wikiers could you please let me know is their any nmr experment to identification of Dimer and tetramer equil...
Justin Frank 1 Jun 29 '16 at 19:09

Beginner: Help understanding 3D NOESY

2 0 6k
answers votes views
Hi, I am a person with a Computer Science background who is dealing with some NMR dataset. I am concerned with the assig...
Justin Frank 1 Jun 27 '16 at 23:13

N15 NNH separated NOESY

2 0 4k
answers votes views
Dear friends, I came across one reference which describe N15 NNH separated NOESY experiment but I am not able to get ...
Justin Frank 1 Jun 27 '16 at 22:53

Protein-Protein Interactions

1 0 2k
answers votes views
Hi i'm a newbie in NMR and I have 2 proteins, A protein is about 12KDa, B protein is about 45 KDa,i know that A protein ...
Justin Frank 1 Jun 27 '16 at 22:46
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