Tagged questions

Will intermediate exchange give poor half filtered NOE

1 1 4k
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I have recorded 2D half filtered NOE experiments on a protein-protein complex and got very poor quality data, why would ...
Posted: TomgA20 61 / Updated: Evgeny Fadeev 5771 Jul 15 '10 at 08:17

How should molecule flexibility affect NOE distance restraint calibration?

1 0 5k
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How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it i...
Updated: Evgeny Fadeev 5771 Mar 13 '10 at 18:48

What software can do relaxation matrix analysis of NOE?

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Hello, could you suggest software to conduct proper relaxation matrix analysis of NOE? Is it available anywhere on the ...
Posted: Evgeny Fadeev 5771 / Updated: sekhar Talluri 621 Feb 13 '10 at 12:43

How to set NOESY mixing time in intermediate tumbling regime

1 0 6k
answers votes views
I need to run noesy for a monomeric peptide of about 1.5kDa at 800 MHz 1H frequency. NOE peaks are weak at 100 ms mixing...
Posted: Evgeny Fadeev 5771 / Updated: sekhar Talluri 621 Feb 13 '10 at 12:00

Select a reasonable NOESY mixing time given MW, B, viscosity?

2 1 6k
answers votes views
Is there a formula that would suggest a reasonable starting value for the mixing time based on the molecular weight, mag...
Updated: Evgeny Fadeev 5771 Feb 12 '10 at 12:32
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