Unanswered questions

How to display NMR structure of molecule with non-standard residues?

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... solving a structure of a cyclic peptide with Ornithine residue, where Orn side-chain participates in an amide bond. ...
Updated: Evgeny Fadeev 5771 Feb 02 '10 at 08:59

Is it possible to record EPR in THF (tetrahydrofuran) at -78C ?

0 0 6k
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We've tried to measure an EPR spectrum of something in THF at -78C and could not tune the cavity on our Bruker EMX500 sp...
Updated: Evgeny Fadeev 5771 Feb 12 '10 at 15:52

Is it safe to do levitation demo with superconducting NMR magnets?

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We do this trick with our 800 MHz Oxford Instruments-built magnet often to impress some folks: Remove the probe and plu...
Posted: Evgeny Fadeev 5771 / Updated: Jerry Hirschinger 199 Sep 28 '10 at 13:53

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

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By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry. Also - ...
Posted: Evgeny Fadeev 5771 / Updated: SankarampadiAravamudhan 91 Mar 17 '11 at 01:01

Are there alternatives to MOLMOL for visualization of NMR structure ensemebles?

2 0 5k
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Hi folks, I've been using Molmol, but found significant issues when working with a non-standard structure. For example...
Updated: Evgeny Fadeev 5771 Mar 02 '10 at 08:05

How do you use PyMol to visualize structure bundles and related MR data?

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Hello, are you using pymol to analyze NMR structures? (I've been mostly using Molmol before). Have you learned any PyMo...
Updated: Evgeny Fadeev 5771 Mar 02 '10 at 12:29

NMRPipe: Why Varian States-TPPI NMR data is to be processed with -yMode Complex?

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Hello, I've started using Varian instrument at UCI two years ago and I use nmrPipe to process the data. This one thing ...
Posted: Evgeny Fadeev 5771 / Updated: Clemens Anklin 416 Jun 06 '10 at 15:14

How do you determine error on the peak position?

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Hello, could anyone suggest a method to determine error (maybe standard deviation or some other estimate) of a peak posi...
Posted: Evgeny Fadeev 5771 / Updated: sekhar Talluri 621 Mar 14 '10 at 08:46

How should molecule flexibility affect NOE distance restraint calibration?

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How would you recommend to approach this problem - we have a peptide that has side-chain exposed to the solvent and it i...
Updated: Evgeny Fadeev 5771 Mar 13 '10 at 18:48

Is there a tool to manage NOE and other restraint lists for the structure calculations?

1 0 4k
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Hello, I'm currently working on solving a peptide NMR solution structure using NIH-XPLOR. I find myself a lot starting ...
Posted: Evgeny Fadeev 5771 / Updated: Ilango 171 Apr 02 '10 at 05:45
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